Plant-derived pectin nanocoatings to prevent inflammatory cellular response of osteoblasts following Porphyromonas gingivalis infection

Research output: Contribution to journalArticlepeer-review

Authors

  • Anna Meresta
  • Justyna Folkert
  • Timo Gaber
  • Korneliusz Miksch
  • Frank Buttgereit
  • Jacqueline Detert
  • Nicole Pischon

Colleges, School and Institutes

External organisations

  • Environmental Biotechnology Department, Faculty of Power and Environmental, Silesian University of Technology, Gliwice, Poland.
  • Department of Medicine, Division of Clinical Immunology and Rheumatology, University of Alabama at Birmingham, Birmingham, AL, USA.
  • Department of Periodontology, Charité University Medicine, Berlin, Germany.
  • Department of Periodontology, Charité University Medicine, Berlin, Germany; Oral Surgery Department, The School of Dentistry, University of Birmingham, Birmingham, UK.

Abstract

BACKGROUND: Bioengineered plant-derived Rhamnogalacturonan-Is (RG-Is) from pectins are potential candidates for surface nanocoating of medical devices. It has recently been reported that RG-I nanocoatings may prevent bacterial infection and improve the biocompatibility of implants. The aim of the study was to evaluate in vitro impact of bioengineered RG-I nanocoatings on osteogenic capacity and proinflammatory cytokine response of murine osteoblasts following Porphyromonas gingivalis infection.

METHODS: Murine MC3T3-E1 osteoblasts and isolated primary calvarial osteoblasts from C57BL/6J (B6J osteoblasts) mice were infected with P. gingivalis and incubated on tissue culture polystyrene plates with or without nanocoatings of unmodified RG-Is isolated from potato pulps (PU) or dearabinanated RG-Is (PA). To investigate a behavior of infected osteoblasts cultured on RG-Is cell morphology, proliferation, metabolic activity, mineralization and osteogenic and pro-inflammatory gene expression were examined.

RESULTS: Following P. gingivalis infection, PA, but not PU, significantly promoted MC3T3-E1 and BJ6 osteoblasts proliferation, metabolic activity, and calcium deposition. Moreover, Il-1b, Il-6, TNF-α, and Rankl gene expressions were downregulated in cells cultured on PU and to a higher extent on PA as compared to the corresponding control, whereas Runx, Alpl, Col1a1, and Bglap gene expressions were upregulated vice versa.

CONCLUSION: Our data clearly showed that pectin RG-Is nanocoating with high content of galactan (PA) reduces the osteoblastic response to P. gingivalis infection in vitro and may, therefore, reduce a risk of inflammation especially in immunocompromised patients with rheumatoid or periodontal disorders.

Details

Original languageEnglish
Pages (from-to)433-445
Number of pages13
JournalInternational Journal of Nanomedicine
Volume12
Publication statusPublished - 12 Jan 2017

Keywords

  • Animals, Cell Proliferation, Cell Shape, Cells, Cultured, Coated Materials, Biocompatible, Gene Expression Regulation, Inflammation, Mice, Inbred C57BL, Nanoparticles, Osteoblasts, Pectins, Porphyromonas gingivalis, Real-Time Polymerase Chain Reaction, Solanum tuberosum, Journal Article