TY - JOUR
T1 - Pivoting of microtubules around the spindle pole accelerates kinetochore capture
AU - Kalinina, Iana
AU - Nandi, Amitabha
AU - Delivani, Petrina
AU - Chacón, Mariola R
AU - Klemm, Anna H
AU - Ramunno-Johnson, Damien
AU - Krull, Alexander
AU - Lindner, Benjamin
AU - Pavin, Nenad
AU - Tolić-Nørrelykke, Iva M
PY - 2012/12/9
Y1 - 2012/12/9
N2 - During cell division, spindle microtubules attach to chromosomes through kinetochores, protein complexes on the chromosome1. The central question is how microtubules find kinetochores. According to the pioneering idea termed search-and-capture, numerous microtubules grow from a centrosome in all directions and by chance capture kinetochores2,3,4. The efficiency of search-and-capture can be improved by a bias in microtubule growth towards the kinetochores5,6, by nucleation of microtubules at the kinetochores7,8,9 and at spindle microtubules10,11, by kinetochore movement9, or by a combination of these processes12,13,14. Here we show in fission yeast that kinetochores are captured by microtubules pivoting around the spindle pole, instead of growing towards the kinetochores. This pivoting motion of microtubules is random and independent of ATP-driven motor activity. By introducing a theoretical model, we show that the measured random movement of microtubules and kinetochores is sufficient to explain the process of kinetochore capture. Our theory predicts that the speed of capture depends mainly on how fast microtubules pivot, which was confirmed experimentally by speeding up and slowing down microtubule pivoting. Thus, pivoting motion allows microtubules to explore space laterally, as they search for targets such as kinetochores.
AB - During cell division, spindle microtubules attach to chromosomes through kinetochores, protein complexes on the chromosome1. The central question is how microtubules find kinetochores. According to the pioneering idea termed search-and-capture, numerous microtubules grow from a centrosome in all directions and by chance capture kinetochores2,3,4. The efficiency of search-and-capture can be improved by a bias in microtubule growth towards the kinetochores5,6, by nucleation of microtubules at the kinetochores7,8,9 and at spindle microtubules10,11, by kinetochore movement9, or by a combination of these processes12,13,14. Here we show in fission yeast that kinetochores are captured by microtubules pivoting around the spindle pole, instead of growing towards the kinetochores. This pivoting motion of microtubules is random and independent of ATP-driven motor activity. By introducing a theoretical model, we show that the measured random movement of microtubules and kinetochores is sufficient to explain the process of kinetochore capture. Our theory predicts that the speed of capture depends mainly on how fast microtubules pivot, which was confirmed experimentally by speeding up and slowing down microtubule pivoting. Thus, pivoting motion allows microtubules to explore space laterally, as they search for targets such as kinetochores.
U2 - 10.1038/ncb2640
DO - 10.1038/ncb2640
M3 - Article
SN - 1465-7392
VL - 15
SP - 82
EP - 87
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 1
ER -