Pigment Epithelium-Derived Factor Promotes Axon Regeneration and Functional Recovery After Spinal Cord Injury

Andrew Stevens; Umar Ahmed; Vasanthy Vigneswara; Zubair Ahmed

doi:10.1007/s12035-019-1614-2

Pigment Epithelium-Derived Factor Promotes Axon Regeneration and Functional Recovery After Spinal Cord Injury

Andrew Stevens, Umar Ahmed, Vasanthy Vigneswara, Zubair Ahmed

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

194 Downloads (Pure)

Abstract

Although neurons in the adult mammalian CNS are inherently incapable of regeneration after injury, we previously showed that exogenous delivery of pigment epithelium-derived factor (PEDF), a 50-kDa neurotrophic factor (NTF), promoted adult retinal ganglion cell neuroprotection and axon regeneration. Here, we show that PEDF and other elements of the PEDF pathway are highly upregulated in dorsal root ganglion neurons (DRGN) from regenerating dorsal column (DC) injury paradigms when compared with non-regenerating DC injury models. Exogenous PEDF was neuroprotective to adult DRGN and disinhibited neurite outgrowth, whilst overexpression of PEDF after DC injury in vivo promoted significant DC axon regeneration with enhanced electrophysiological, sensory, and locomotor function. Our findings reveal that PEDF is a novel NTF for adult DRGN and may represent a therapeutically useful factor to promote functional recovery after spinal cord injury.

Original language	English
Pages (from-to)	7490-7507
Number of pages	18
Journal	Molecular Neurobiology
Volume	56
Issue number	11
Early online date	2 May 2019
DOIs	https://doi.org/10.1007/s12035-019-1614-2
Publication status	Published - Nov 2019

Keywords

PEDF
Spinal cord injury
Serum withdrawal
Dorsal root ganglia neurons
CNS
Axon regeneration
Neurite outgrowth

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10.1007/s12035-019-1614-2Licence: Creative Commons: Attribution (CC BY)

Stevens_et_al_2019_Pigment_epithelium-Derived_factor_Molecular_NeurobiologyFinal published version, 3.6 MBLicence: Creative Commons: Attribution (CC BY)

The Role of Caspase-2 in Retinal Ganglion Cell Apoptosis After Optic Nerve Transection
Ahmed, Z.
THE WELLCOME TRUST
1/12/10 → 30/11/13
Project: Research

Cite this

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title = "Pigment Epithelium-Derived Factor Promotes Axon Regeneration and Functional Recovery After Spinal Cord Injury",

abstract = "Although neurons in the adult mammalian CNS are inherently incapable of regeneration after injury, we previously showed that exogenous delivery of pigment epithelium-derived factor (PEDF), a 50-kDa neurotrophic factor (NTF), promoted adult retinal ganglion cell neuroprotection and axon regeneration. Here, we show that PEDF and other elements of the PEDF pathway are highly upregulated in dorsal root ganglion neurons (DRGN) from regenerating dorsal column (DC) injury paradigms when compared with non-regenerating DC injury models. Exogenous PEDF was neuroprotective to adult DRGN and disinhibited neurite outgrowth, whilst overexpression of PEDF after DC injury in vivo promoted significant DC axon regeneration with enhanced electrophysiological, sensory, and locomotor function. Our findings reveal that PEDF is a novel NTF for adult DRGN and may represent a therapeutically useful factor to promote functional recovery after spinal cord injury.",

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Y1 - 2019/11

N2 - Although neurons in the adult mammalian CNS are inherently incapable of regeneration after injury, we previously showed that exogenous delivery of pigment epithelium-derived factor (PEDF), a 50-kDa neurotrophic factor (NTF), promoted adult retinal ganglion cell neuroprotection and axon regeneration. Here, we show that PEDF and other elements of the PEDF pathway are highly upregulated in dorsal root ganglion neurons (DRGN) from regenerating dorsal column (DC) injury paradigms when compared with non-regenerating DC injury models. Exogenous PEDF was neuroprotective to adult DRGN and disinhibited neurite outgrowth, whilst overexpression of PEDF after DC injury in vivo promoted significant DC axon regeneration with enhanced electrophysiological, sensory, and locomotor function. Our findings reveal that PEDF is a novel NTF for adult DRGN and may represent a therapeutically useful factor to promote functional recovery after spinal cord injury.

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Pigment Epithelium-Derived Factor Promotes Axon Regeneration and Functional Recovery After Spinal Cord Injury

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Keywords

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The Role of Caspase-2 in Retinal Ganglion Cell Apoptosis After Optic Nerve Transection

Cite this