Pigment Epithelium-Derived Factor Promotes Axon Regeneration and Functional Recovery After Spinal Cord Injury

Research output: Contribution to journalArticle

External organisations

  • Neuroscience and Ophthalmology, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, Robert Aitken Institute of Clinical Research, University of Birmingham, Birmingham, B15 2TT, UK.
  • King Edward VI Camp Hill School for Boys, Vicarage Road, Kings Heath, Birmingham, B14 7QJ, UK.
  • Neuroscience and Ophthalmology, Institute of Inflammation and Ageing, College of Medical and Dental Sciences, Robert Aitken Institute of Clinical Research, University of Birmingham, Birmingham, B15 2TT, UK. z.ahmed.1@bham.ac.uk.

Abstract

Although neurons in the adult mammalian CNS are inherently incapable of regeneration after injury, we previously showed that exogenous delivery of pigment epithelium-derived factor (PEDF), a 50-kDa neurotrophic factor (NTF), promoted adult retinal ganglion cell neuroprotection and axon regeneration. Here, we show that PEDF and other elements of the PEDF pathway are highly upregulated in dorsal root ganglion neurons (DRGN) from regenerating dorsal column (DC) injury paradigms when compared with non-regenerating DC injury models. Exogenous PEDF was neuroprotective to adult DRGN and disinhibited neurite outgrowth, whilst overexpression of PEDF after DC injury in vivo promoted significant DC axon regeneration with enhanced electrophysiological, sensory, and locomotor function. Our findings reveal that PEDF is a novel NTF for adult DRGN and may represent a therapeutically useful factor to promote functional recovery after spinal cord injury.

Details

Original languageEnglish
JournalMolecular Neurobiology
Early online date2 May 2019
Publication statusE-pub ahead of print - 2 May 2019

Keywords

  • PEDF, Spinal cord injury, Serum withdrawal, Dorsal root ganglia neurons, CNS, Axon regeneration, Neurite outgrowth