Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles

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Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles. / Varlas, Spyridon; Blackman, Lewis D.; Findlay, Heather E.; Reading, Eamonn; Booth, Paula J.; Gibson, Matthew I.; O'Reilly, Rachel K.

In: Macromolecules, Vol. 51, No. 16, 28.08.2018, p. 6190-6201.

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Varlas, Spyridon ; Blackman, Lewis D. ; Findlay, Heather E. ; Reading, Eamonn ; Booth, Paula J. ; Gibson, Matthew I. ; O'Reilly, Rachel K. / Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles. In: Macromolecules. 2018 ; Vol. 51, No. 16. pp. 6190-6201.

Bibtex

@article{4df6d4eb85b9437ea5db69dc0caa6a46,
title = "Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles",
abstract = "Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of morphologies. Given that this process can be performed at high concentrations and under mild reaction conditions, it has the potential to have significant industrial scope. However, given that the majority of industrial (and more specifically biotechnological) formulations contain mixtures of polymers and surfactants, the effect of such surfactants on the PISA process is an important consideration. Thus, to expand the scope of the methodology, the effect of small molecule surfactants on the PISA process, specifically for the preparation of unilamellar vesicles, was investigated. Similar to aqueous photo-PISA findings in the absence of surfactant molecules, the originally targeted vesicular morphology was retained in the presence of varying concentrations of non-ionic surfactants, while a diverse set of lower-order morphologies was observed for ionic surfactants. Interestingly, a critical micelle concentration (CMC)-dependent behavior was detected in the case of zwitterionic detergents. Additionally, tunable size and membrane thickness of vesicles were observed by using different types and concentration of surfactants. Based on these findings, a functional channel-forming membrane protein (OmpF porin), stabilized in aqueous media by surfactant molecules, was able to be directly inserted into the membrane of vesicles during photo-PISA. Our study demonstrates the potential of photo-PISA for the direct formation of protein-polymer complexes and highlights how this method could be used to design biomimicking polymer/surfactant nanoreactors.",
author = "Spyridon Varlas and Blackman, {Lewis D.} and Findlay, {Heather E.} and Eamonn Reading and Booth, {Paula J.} and Gibson, {Matthew I.} and O'Reilly, {Rachel K.}",
year = "2018",
month = aug,
day = "28",
doi = "10.1021/acs.macromol.8b00994",
language = "English",
volume = "51",
pages = "6190--6201",
journal = "Macromolecules",
issn = "0024-9297",
publisher = "American Chemical Society",
number = "16",

}

RIS

TY - JOUR

T1 - Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles

AU - Varlas, Spyridon

AU - Blackman, Lewis D.

AU - Findlay, Heather E.

AU - Reading, Eamonn

AU - Booth, Paula J.

AU - Gibson, Matthew I.

AU - O'Reilly, Rachel K.

PY - 2018/8/28

Y1 - 2018/8/28

N2 - Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of morphologies. Given that this process can be performed at high concentrations and under mild reaction conditions, it has the potential to have significant industrial scope. However, given that the majority of industrial (and more specifically biotechnological) formulations contain mixtures of polymers and surfactants, the effect of such surfactants on the PISA process is an important consideration. Thus, to expand the scope of the methodology, the effect of small molecule surfactants on the PISA process, specifically for the preparation of unilamellar vesicles, was investigated. Similar to aqueous photo-PISA findings in the absence of surfactant molecules, the originally targeted vesicular morphology was retained in the presence of varying concentrations of non-ionic surfactants, while a diverse set of lower-order morphologies was observed for ionic surfactants. Interestingly, a critical micelle concentration (CMC)-dependent behavior was detected in the case of zwitterionic detergents. Additionally, tunable size and membrane thickness of vesicles were observed by using different types and concentration of surfactants. Based on these findings, a functional channel-forming membrane protein (OmpF porin), stabilized in aqueous media by surfactant molecules, was able to be directly inserted into the membrane of vesicles during photo-PISA. Our study demonstrates the potential of photo-PISA for the direct formation of protein-polymer complexes and highlights how this method could be used to design biomimicking polymer/surfactant nanoreactors.

AB - Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of morphologies. Given that this process can be performed at high concentrations and under mild reaction conditions, it has the potential to have significant industrial scope. However, given that the majority of industrial (and more specifically biotechnological) formulations contain mixtures of polymers and surfactants, the effect of such surfactants on the PISA process is an important consideration. Thus, to expand the scope of the methodology, the effect of small molecule surfactants on the PISA process, specifically for the preparation of unilamellar vesicles, was investigated. Similar to aqueous photo-PISA findings in the absence of surfactant molecules, the originally targeted vesicular morphology was retained in the presence of varying concentrations of non-ionic surfactants, while a diverse set of lower-order morphologies was observed for ionic surfactants. Interestingly, a critical micelle concentration (CMC)-dependent behavior was detected in the case of zwitterionic detergents. Additionally, tunable size and membrane thickness of vesicles were observed by using different types and concentration of surfactants. Based on these findings, a functional channel-forming membrane protein (OmpF porin), stabilized in aqueous media by surfactant molecules, was able to be directly inserted into the membrane of vesicles during photo-PISA. Our study demonstrates the potential of photo-PISA for the direct formation of protein-polymer complexes and highlights how this method could be used to design biomimicking polymer/surfactant nanoreactors.

UR - http://www.scopus.com/inward/record.url?scp=85052588047&partnerID=8YFLogxK

U2 - 10.1021/acs.macromol.8b00994

DO - 10.1021/acs.macromol.8b00994

M3 - Article

AN - SCOPUS:85052588047

VL - 51

SP - 6190

EP - 6201

JO - Macromolecules

JF - Macromolecules

SN - 0024-9297

IS - 16

ER -