Photoactive platinum(IV) complex conjugated to a cancer-cell-targeting cyclic peptide

Research output: Contribution to journalArticlepeer-review

Authors

  • Huayun Shi
  • Qian Wang
  • V Venkatesh
  • Guokai Feng
  • Lawrence S Young
  • Musheng Zeng
  • Peter J. Sadler

Colleges, School and Institutes

Abstract

A conjugate of cancer-cell targeting cyclic disulphide nona-peptide c(CRWYDENAC) consisting of nine l-amino acids with the photoactive succinate platinum(iv) complex trans,trans-[Pt(N 3) 2(py) 2(OH)(succinate)] (Pt-cP) has been synthesised and characterised. The conjugate was stable in dark, but released succinate-peptide and Pt(ii) species upon irradiation with visible light, and formed photoproducts with guanine. Conjugate Pt-cP exhibited higher photocytotoxicity than parent complex trans,trans,trans-[Pt(N 3) 2(OH) 2(py) 2] (FM-190) towards cancer cells, including ovarian A2780, lung A549 and prostate PC3 human cancer cells upon irradiation with blue light (465 nm, 17.28 J cm -2) with IC 50 values of 2.8-22.4 μM and the highest potency for A549 cells. Even though the dark cellular accumulation of Pt-cP in A2780 cells was lower than that of parent FM-190, Pt from Pt-cP accumulated in cancer cells upon irradiation to a level >3× higher than that from FM-190. In addition, the cellular accumulation of Pt from Pt-cP was enhanced ca. 47× after irradiation.

Details

Original languageEnglish
Pages (from-to)8560-8564
Number of pages5
JournalDalton Transactions
Volume48
Issue number24
Early online date31 May 2019
Publication statusPublished - 6 Aug 2019