pH dependent binding in de novo hetero bimetallic coiled coils

Research output: Contribution to journalArticle

Standard

pH dependent binding in de novo hetero bimetallic coiled coils. / Teare, Paul; Smith, Caitlin F; Adams, Samuel J; Anbu, Sellamuthu; Ciani, Barbara; Jeuken, Lars J C; Peacock, Anna F A.

In: Dalton Transactions, Vol. 47, No. 31, 21.08.2018, p. 10784-10790.

Research output: Contribution to journalArticle

Harvard

Teare, P, Smith, CF, Adams, SJ, Anbu, S, Ciani, B, Jeuken, LJC & Peacock, AFA 2018, 'pH dependent binding in de novo hetero bimetallic coiled coils', Dalton Transactions, vol. 47, no. 31, pp. 10784-10790. https://doi.org/10.1039/c8dt01568f

APA

Teare, P., Smith, C. F., Adams, S. J., Anbu, S., Ciani, B., Jeuken, L. J. C., & Peacock, A. F. A. (2018). pH dependent binding in de novo hetero bimetallic coiled coils. Dalton Transactions, 47(31), 10784-10790. https://doi.org/10.1039/c8dt01568f

Vancouver

Teare P, Smith CF, Adams SJ, Anbu S, Ciani B, Jeuken LJC et al. pH dependent binding in de novo hetero bimetallic coiled coils. Dalton Transactions. 2018 Aug 21;47(31):10784-10790. https://doi.org/10.1039/c8dt01568f

Author

Teare, Paul ; Smith, Caitlin F ; Adams, Samuel J ; Anbu, Sellamuthu ; Ciani, Barbara ; Jeuken, Lars J C ; Peacock, Anna F A. / pH dependent binding in de novo hetero bimetallic coiled coils. In: Dalton Transactions. 2018 ; Vol. 47, No. 31. pp. 10784-10790.

Bibtex

@article{317c5a82b8284e3e80f9368073f15d0a,
title = "pH dependent binding in de novo hetero bimetallic coiled coils",
abstract = "Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(ii) and Tb(iii) to the two distinct sites within this coiled coil, and offers the opportunity to {"}activate{"} metal binding sites within complex multi metallo and multi-functional designs.",
author = "Paul Teare and Smith, {Caitlin F} and Adams, {Samuel J} and Sellamuthu Anbu and Barbara Ciani and Jeuken, {Lars J C} and Peacock, {Anna F A}",
year = "2018",
month = "8",
day = "21",
doi = "10.1039/c8dt01568f",
language = "English",
volume = "47",
pages = "10784--10790",
journal = "Dalton Transactions",
issn = "1477-9234",
publisher = "Royal Society of Chemistry",
number = "31",

}

RIS

TY - JOUR

T1 - pH dependent binding in de novo hetero bimetallic coiled coils

AU - Teare, Paul

AU - Smith, Caitlin F

AU - Adams, Samuel J

AU - Anbu, Sellamuthu

AU - Ciani, Barbara

AU - Jeuken, Lars J C

AU - Peacock, Anna F A

PY - 2018/8/21

Y1 - 2018/8/21

N2 - Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(ii) and Tb(iii) to the two distinct sites within this coiled coil, and offers the opportunity to "activate" metal binding sites within complex multi metallo and multi-functional designs.

AB - Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(ii) and Tb(iii) to the two distinct sites within this coiled coil, and offers the opportunity to "activate" metal binding sites within complex multi metallo and multi-functional designs.

U2 - 10.1039/c8dt01568f

DO - 10.1039/c8dt01568f

M3 - Article

C2 - 30022210

VL - 47

SP - 10784

EP - 10790

JO - Dalton Transactions

JF - Dalton Transactions

SN - 1477-9234

IS - 31

ER -