pH dependent binding in de novo hetero bimetallic coiled coils

Research output: Contribution to journalArticle

Authors

  • Paul Teare
  • Caitlin F Smith
  • Samuel J Adams
  • Sellamuthu Anbu
  • Barbara Ciani
  • Lars J C Jeuken

Colleges, School and Institutes

External organisations

  • School of Chemistry, University of Birmingham, Edgbaston, B15 2TT, UK. a.f.a.peacock@bham.ac.uk.
  • Centre for Membrane Interactions and Dynamics, and Krebs Institute, Department of Chemistry, University of Sheffield, Sheffield, S3 7HF, UK.
  • University of Leeds

Abstract

Herein the first example of a bimetallic coiled coil featuring a lanthanide binding site is reported, opening opportunities to exploit the attractive NMR and photophysical properties of the lanthanides in multi metallo protein design. In our efforts to fully characterise the system we identified for the first time that lanthanide binding to such sites is pH dependent, with optimal binding at neutral pH, and that the double AsnAsp site is more versatile in this regard than the single Asp site. Our second site featured the structural HgCys3 site, the chemistry of which was essentially unaltered by the presence of the lanthanide site. In fact, both metal binding sites within the hetero bimetallic coiled coil displayed the same properties as their mononuclear single binding site controls, and operated independently of each other. Finally, pH can be used as an external trigger to control the binding of Hg(ii) and Tb(iii) to the two distinct sites within this coiled coil, and offers the opportunity to "activate" metal binding sites within complex multi metallo and multi-functional designs.

Details

Original languageEnglish
Pages (from-to)10784-10790
Number of pages7
JournalDalton Transactions
Volume47
Issue number31
Early online date5 Jul 2018
Publication statusPublished - 21 Aug 2018