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Abstract
The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry.
Original language | English |
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Pages (from-to) | 1369-1373 |
Number of pages | 5 |
Journal | Journal of General Virology |
Volume | 96 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1 Jun 2015 |
Bibliographical note
© 2015 The Authors.Keywords
- Cells, Cultured
- Hepacivirus
- Hepatocytes
- Humans
- Viral Tropism
- Virus Internalization
- Virus Replication
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Dive into the research topics of 'Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection'. Together they form a unique fingerprint.Projects
- 2 Finished
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The Role of Hepatitis C Virus Glycoprotein-Receptor Polymorphism in Viral Pathogenesis
McKeating, J.
1/01/12 → 30/06/17
Project: Research Councils
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Mechanisms of Hepatitis C Virus Induced Hepatocyte Injury
McKeating, J. & Balfe, P.
1/10/09 → 30/09/12
Project: Research Councils