Peripheral lymph nodes contain migratory and resident innate lymphoid cell populations

Research output: Contribution to journalArticle

Authors

  • Emma E Dutton
  • Dominika W Gajdasik
  • Claire Willis
  • Emma L Bishop
  • Ana Camelo
  • Matthew A Sleeman
  • Margherita Coccia
  • Arnaud M Didierlaurent
  • Michio Tomura
  • Fernanda Pilataxi
  • Christopher A Morehouse
  • Gianluca Carlesso

External organisations

  • Institute of Immunology & Immunotherapy; College of Medical and Dental Sciences; University of Birmingham; Birmingham UK
  • MedImmune LLC, Aaron Klug building, Granta Park, Cambridge CB21 6GH, UK.
  • GSK, Rue de l'Institut 89, 1330 Rixensart, Belgium.
  • Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiorikita, Tondabayashi City, Osaka Prefecture, 584-8540, Japan.
  • Department of Translational Medicine-Pharmacogenomics, MedImmune LLC, Gaithersburg, MD 20878, USA.
  • Department of Translational Medicine Research, MedImmune LLC, Gaithersburg, MD 20878, USA.
  • Department of Cancer Biology, MedImmune LLC, Gaithersburg, MD 20878, USA.
  • Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK. d.withers@bham.ac.uk.

Abstract

Tissue residency is considered a defining feature of the innate lymphoid cell (ILC) populations located within mucosal and adipose tissues. ILCs are also present within all lymphoid tissues, but whether ILCs migrate between lymphoid and nonlymphoid sites and in what context is poorly understood. To determine whether migratory ILCs exist within peripheral lymph nodes (LNs), we labeled all cells within the brachial LN (bLN) of transgenic mice expressing a photoconvertible fluorescent protein by direct exposure to light. Tracking of cellular changes in the labeled LN revealed the gradual migration of new ILCs into the tissue, balanced by egress of ILCs dependent on sphingosine-1-phosphate receptors. Most of the migratory ILCs were ILC1s, entering LNs directly from the circulation in a CD62L- and CCR7-dependent manner and thus behaving like conventional natural killer (cNK) cells. Upon egress, both ILC1s and cNK cells were found to recirculate through peripheral LNs. A distinct population of migratory ILC2s were detected in the LN, but most of the ILC3s were tissue resident. Functionally, both migratory and resident ILC1s within LNs were able to rapidly produce IFN-γ to support the generation of robust TH1 T cell responses after immunization. Thus, migratory and resident ILC populations exist within peripheral LNs, with ILC1s, akin to cNK cells, able to traffic into these tissues where they can contribute to the initiation of adaptive immunity.

Bibliographic note

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Details

Original languageEnglish
Article numbereaau8082
JournalScience Immunology
Volume4
Issue number35
Publication statusPublished - 31 May 2019

ASJC Scopus subject areas