Pectin nanocoating of titanium implant surfaces - an experimental study in rabbits

Research output: Contribution to journalArticle

Authors

  • Kai Dirscherl
  • Bodil Jørgensen
  • Tord Berglundh
  • Niklas Rye Jørgensen
  • Klaus Gotfredsen

Colleges, School and Institutes

External organisations

  • Institute of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen N, Denmark.
  • Danish National Metrology Institute, Lyngby, Denmark.
  • Department of Plant and Environmental Sciences, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C, Denmark.
  • Department of Periodontolgy, Institute of Odontology, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
  • Research Center for Ageing and Osteoporosis, Departments of Diagnostics and Medicine and Clinical Biochemistry, Copenhagen University Hospital Glostrup, Glostrup, Denmark.

Abstract

INTRODUCTION: A major determinant of successful osseointegration of endosseous implants is the surface of the implant, which influences the cellular response of the surrounding tissues. A new strategy to improve osseointegration and bone healing is biochemical stimulation by surface nanocoatings that may increase adhesion of bone proteins, and bone cells at the implant surface. Nanocoating with pectins, plant cell wall-derived polysaccharides, is frequently done using rhamnogalacturonan-I (RG-I).

AIM: The aim of the study was to evaluate the effect of nanocoating titanium implants with plant cell wall-derived rhamnogalacturonan-I, on bone healing and osseointegration.

MATERIAL AND METHODS: Machined titanium implants were coated with three modifications of rhamnogalacturonan-I (RG-I). Chemical and physical surface properties were examined before insertion of nanocoated implants (n = 96) into the left and right tibia of rabbits. Machined titanium implants without RG-I nanocoating were used as controls (n = 32). Total number of 128 implants was placed in tibias of 16 rabbits. Fluorochrome bone labels, calcein green and alizarin red S were given intravenously after 9 and 12 days, respectively. The bone response to the nanocoated implants was analyzed qualitatively and quantitatively after 2, 4, 6, and 8 weeks of healing using light microscopy and histomorphometric methods.

RESULTS: The RG-I coating influenced the surface chemical composition; wettability and roughness, making the surface more hydrophilic without any major effect on surface micro roughness compared to control implant surfaces. The different modifications of pectin RG-I did not significantly enhance bone healing and osseointegration analyzed after 2, 4, 6, and 8 weeks of healing compared to control implants. Although the qualitative analyses of the fluorochromes indicated a higher activity of bone formation in the mineralization front at the early stage, after 9 and 12 days at the RG-I nanocoated implants compared to the control implants although no significant quantitative difference was demonstrated.

CONCLUSION: The present study showed that nanocoating of titanium implants with pectin RG-Is did not significantly enhance bone healing and osseointegration when placed in rabbit tibia bone.

Details

Original languageEnglish
Pages (from-to)298-307
Number of pages10
JournalClinical Oral Implants Research
Volume28
Issue number3
Early online date14 Mar 2016
Publication statusPublished - Mar 2017

Keywords

  • Journal Article