Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat

Research output: Contribution to journalArticle

Authors

  • Eric J Belfield
  • Caifu Jiang
  • Carly Brown
  • Aziz Mithani
  • Nicholas P Harberd

Colleges, School and Institutes

Abstract

Bread wheat (Triticum aestivum) has a large, complex and hexaploid genome consisting of A, B and D homoeologous chromosome sets. Therefore each wheat gene potentially exists as a trio of A, B and D homoeoloci, each of which may contribute differentially to wheat phenotypes. We describe a novel approach combining wheat cytogenetic resources (chromosome substitution 'nullisomic-tetrasomic' lines) with next generation deep sequencing of gene transcripts (RNA-Seq), to directly and accurately identify homoeologue-specific single nucleotide variants and quantify the relative contribution of individual homoeoloci to gene expression.

Details

Original languageEnglish
Article number276
JournalBMC Genomics
Volume15
Publication statusPublished - 11 Apr 2014