Projects per year
Abstract
OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.
METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.
RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.
CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.
Original language | English |
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Pages (from-to) | 2241-2248 |
Journal | Journal of Antimicrobial Chemotherapy |
Volume | 70 |
Issue number | 8 |
Early online date | 7 May 2015 |
DOIs | |
Publication status | Published - Aug 2015 |
Bibliographical note
© The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.Keywords
- disinfectant
- MDR
- efflux
Fingerprint
Dive into the research topics of 'Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure'. Together they form a unique fingerprint.Projects
- 3 Finished
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Development of robust analytical pipelines for the analysis of microbial community data from clinical samples
1/09/12 → 31/08/15
Project: Research Councils
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Evolution of Multidrug Resistance in Salmonella Enterica Serovar Typhimurium as a Result of Biocide Exposure
Webber, M., Pallen, M. & Piddock, L.
Biotechnology & Biological Sciences Research Council
13/07/09 → 12/07/12
Project: Research Councils
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xBASE: A bioinformatics resource for the AgriFood Bacteriology Community
Pallen, M. & Stekel, D.
Biotechnology & Biological Sciences Research Council
1/02/07 → 31/01/12
Project: Research Councils