Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure
Research output: Contribution to journal › Article
- Antimicrobials Research Group, School of Immunity and Infection and Institute for Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham
OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance.
METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance.
RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan.
CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.
|Journal||Journal of Antimicrobial Chemotherapy|
|Early online date||7 May 2015|
|Publication status||Published - Aug 2015|
- disinfectant, MDR, efflux