PAP-LMPCR: an improved, sequence-selective method for the in vivo analysis of transcription factor occupancy and chromatin fine structure

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

In vivo footprinting and ligation-mediated PCR (LMPCR) are well-established methods for the examination of the chromatin structure of eukaryotic genes. Here, we describe an improved method (pyrophosphorolysis activated polymerization LMPCR or PAP-LMPCR) that overcomes the shortfalls of previous methods by being capable of reading through sequences that up to now were refractory to this type of analysis. This includes dinucleotide repeat sequences or GC-rich regions. We also describe conditions capable of distinguishing between different alleles, thus enabling the simultaneous analysis of monoallelically expressed genes without having to employ interspecies hybrids.

Details

Original languageEnglish
Pages (from-to)177-92
Number of pages16
JournalMethods in molecular biology
Volume687
Publication statusPublished - 1 Jan 2011

Keywords

  • PAP-LMPCR, chromatin fine structure