Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy

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@article{b15b5c5c71ec4a689ee1c357ea55233d,
title = "Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy",
abstract = "Askwith T, Zeng W, Eggo MC, Stevens MJ. Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy. Am J Physiol Endocrinol Metab 297: E620-E628, 2009. First published July 14, 2009; doi: 10.1152/ajpendo.00287.2009.-In human Schwann cells, the role of taurine in regulating glucose-induced changes in antioxidant defense systems has been examined. Treatment with high glucose for 7 days induced reactive oxygen species, increased 4-hydroxynoneal adducts (20 +/- 5%, P <0.05) and poly(ADP-ribosyl)ated proteins (40 +/- 13%, P <0.05). Increases in these markers of oxidative stress were reversed by simultaneous incubation in 0.25 mM taurine. Both high glucose and taurine independently increased superoxide dismutase and catalase activity and decreased glutathione levels, but their effects were not additive. Glucose reduced taurine transporter (TauT) mRNA and protein in a dose-dependent manner with maximal decreases of 66 +/- 6 and 63 +/- 12%, respectively (P <0.05 both). The V-max for taurine uptake was decreased in 30 mM glucose from 61 +/- 5 to 42 +/- 3 pmol.min(-1).mg protein(-1) (P <0.001). Glucose-induced TauT downregulation could be reversed by inhibition of aldose reductase, a pathway that depletes NADPH and increases osmotic stress and protein glycation. TauT protein was increased more than threefold, and the V-max for taurine uptake doubled (P <0.05 both) by prooxidants. TauT downregulation was reversed both by treatment with the antioxidant alpha-lipoic acid, which increased TauT mRNA by 60% and V-max by 50% (P <0.05 both), and by the aldose reductase inhibitor sorbinil, which increased TauT mRNA 380% and V-max by 98% (P <0.01 both). These data highlight the potential therapeutic benefits of taurine supplementation in diabetic complications and provide mechanisms whereby taurine restoration could be achieved in order to prevent or reverse diabetic complications.",
author = "T Askwith and Wei Zeng and Margaret Eggo and Martin Stevens",
year = "2009",
month = sep,
day = "1",
doi = "10.1152/ajpendo.00287.2009",
language = "English",
volume = "297",
pages = "E620--E628",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "3",

}

RIS

TY - JOUR

T1 - Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy

AU - Askwith, T

AU - Zeng, Wei

AU - Eggo, Margaret

AU - Stevens, Martin

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Askwith T, Zeng W, Eggo MC, Stevens MJ. Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy. Am J Physiol Endocrinol Metab 297: E620-E628, 2009. First published July 14, 2009; doi: 10.1152/ajpendo.00287.2009.-In human Schwann cells, the role of taurine in regulating glucose-induced changes in antioxidant defense systems has been examined. Treatment with high glucose for 7 days induced reactive oxygen species, increased 4-hydroxynoneal adducts (20 +/- 5%, P <0.05) and poly(ADP-ribosyl)ated proteins (40 +/- 13%, P <0.05). Increases in these markers of oxidative stress were reversed by simultaneous incubation in 0.25 mM taurine. Both high glucose and taurine independently increased superoxide dismutase and catalase activity and decreased glutathione levels, but their effects were not additive. Glucose reduced taurine transporter (TauT) mRNA and protein in a dose-dependent manner with maximal decreases of 66 +/- 6 and 63 +/- 12%, respectively (P <0.05 both). The V-max for taurine uptake was decreased in 30 mM glucose from 61 +/- 5 to 42 +/- 3 pmol.min(-1).mg protein(-1) (P <0.001). Glucose-induced TauT downregulation could be reversed by inhibition of aldose reductase, a pathway that depletes NADPH and increases osmotic stress and protein glycation. TauT protein was increased more than threefold, and the V-max for taurine uptake doubled (P <0.05 both) by prooxidants. TauT downregulation was reversed both by treatment with the antioxidant alpha-lipoic acid, which increased TauT mRNA by 60% and V-max by 50% (P <0.05 both), and by the aldose reductase inhibitor sorbinil, which increased TauT mRNA 380% and V-max by 98% (P <0.01 both). These data highlight the potential therapeutic benefits of taurine supplementation in diabetic complications and provide mechanisms whereby taurine restoration could be achieved in order to prevent or reverse diabetic complications.

AB - Askwith T, Zeng W, Eggo MC, Stevens MJ. Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy. Am J Physiol Endocrinol Metab 297: E620-E628, 2009. First published July 14, 2009; doi: 10.1152/ajpendo.00287.2009.-In human Schwann cells, the role of taurine in regulating glucose-induced changes in antioxidant defense systems has been examined. Treatment with high glucose for 7 days induced reactive oxygen species, increased 4-hydroxynoneal adducts (20 +/- 5%, P <0.05) and poly(ADP-ribosyl)ated proteins (40 +/- 13%, P <0.05). Increases in these markers of oxidative stress were reversed by simultaneous incubation in 0.25 mM taurine. Both high glucose and taurine independently increased superoxide dismutase and catalase activity and decreased glutathione levels, but their effects were not additive. Glucose reduced taurine transporter (TauT) mRNA and protein in a dose-dependent manner with maximal decreases of 66 +/- 6 and 63 +/- 12%, respectively (P <0.05 both). The V-max for taurine uptake was decreased in 30 mM glucose from 61 +/- 5 to 42 +/- 3 pmol.min(-1).mg protein(-1) (P <0.001). Glucose-induced TauT downregulation could be reversed by inhibition of aldose reductase, a pathway that depletes NADPH and increases osmotic stress and protein glycation. TauT protein was increased more than threefold, and the V-max for taurine uptake doubled (P <0.05 both) by prooxidants. TauT downregulation was reversed both by treatment with the antioxidant alpha-lipoic acid, which increased TauT mRNA by 60% and V-max by 50% (P <0.05 both), and by the aldose reductase inhibitor sorbinil, which increased TauT mRNA 380% and V-max by 98% (P <0.01 both). These data highlight the potential therapeutic benefits of taurine supplementation in diabetic complications and provide mechanisms whereby taurine restoration could be achieved in order to prevent or reverse diabetic complications.

U2 - 10.1152/ajpendo.00287.2009

DO - 10.1152/ajpendo.00287.2009

M3 - Article

C2 - 19602579

VL - 297

SP - E620-E628

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 3

ER -