OX40 controls effector CD4+ T-cell expansion, not follicular T helper cell generation in acute Listeria infection: Immunomodulation

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OX40 controls effector CD4+ T-cell expansion, not follicular T helper cell generation in acute Listeria infection : Immunomodulation. / Marriott, Clare L.; Mackley, Emma C.; Ferreira, Cristina; Veldhoen, Marc; Yagita, Hideo; Withers, David R.

In: European Journal of Immunology, Vol. 44, No. 8, 01.08.2014, p. 2437-2447.

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Marriott, Clare L. ; Mackley, Emma C. ; Ferreira, Cristina ; Veldhoen, Marc ; Yagita, Hideo ; Withers, David R. / OX40 controls effector CD4+ T-cell expansion, not follicular T helper cell generation in acute Listeria infection : Immunomodulation. In: European Journal of Immunology. 2014 ; Vol. 44, No. 8. pp. 2437-2447.

Bibtex

@article{28ae6d8ba88a4fc69530c22e96ed0d38,
title = "OX40 controls effector CD4+ T-cell expansion, not follicular T helper cell generation in acute Listeria infection: Immunomodulation",
abstract = "To investigate the importance of OX40 signals for physiological CD4(+) T-cell responses, an endogenous antigen-specific population of CD4(+) T cells that recognise the 2W1S peptide was assessed and temporal control of OX40 signals was achieved using blocking or agonistic antibodies (Abs) in vivo. Following infection with Listeria monocytogenes expressing 2W1S peptide, OX40 was briefly expressed by the responding 2W1S-specific CD4(+) T cells, but only on a subset that co-expressed effector cell markers. This population was specifically expanded by Ab-ligation of OX40 during priming, which also caused skewing of the memory response towards effector memory cells. Strikingly, this greatly enhanced effector response was accompanied by the loss of T follicular helper (TFH) cells and germinal centres. Mice deficient in OX40 and CD30 showed normal generation of TFH cells but impaired numbers of 2W1S-specific effector cells. OX40 was not expressed by 2W1S-specific memory cells, although it was rapidly up-regulated upon challenge whereupon Ab-ligation of OX40 specifically affected the effector subset. In summary, these data indicate that for CD4(+) T cells, OX40 signals are important for generation of effector T cells rather than TFH cells in this response to acute bacterial infection.",
keywords = "CD4+ T cells, Costimulation, Germinal centre, Memory, OX40",
author = "Marriott, {Clare L.} and Mackley, {Emma C.} and Cristina Ferreira and Marc Veldhoen and Hideo Yagita and Withers, {David R.}",
year = "2014",
month = aug,
day = "1",
doi = "10.1002/eji.v44.8",
language = "English",
volume = "44",
pages = "2437--2447",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "8",

}

RIS

TY - JOUR

T1 - OX40 controls effector CD4+ T-cell expansion, not follicular T helper cell generation in acute Listeria infection

T2 - Immunomodulation

AU - Marriott, Clare L.

AU - Mackley, Emma C.

AU - Ferreira, Cristina

AU - Veldhoen, Marc

AU - Yagita, Hideo

AU - Withers, David R.

PY - 2014/8/1

Y1 - 2014/8/1

N2 - To investigate the importance of OX40 signals for physiological CD4(+) T-cell responses, an endogenous antigen-specific population of CD4(+) T cells that recognise the 2W1S peptide was assessed and temporal control of OX40 signals was achieved using blocking or agonistic antibodies (Abs) in vivo. Following infection with Listeria monocytogenes expressing 2W1S peptide, OX40 was briefly expressed by the responding 2W1S-specific CD4(+) T cells, but only on a subset that co-expressed effector cell markers. This population was specifically expanded by Ab-ligation of OX40 during priming, which also caused skewing of the memory response towards effector memory cells. Strikingly, this greatly enhanced effector response was accompanied by the loss of T follicular helper (TFH) cells and germinal centres. Mice deficient in OX40 and CD30 showed normal generation of TFH cells but impaired numbers of 2W1S-specific effector cells. OX40 was not expressed by 2W1S-specific memory cells, although it was rapidly up-regulated upon challenge whereupon Ab-ligation of OX40 specifically affected the effector subset. In summary, these data indicate that for CD4(+) T cells, OX40 signals are important for generation of effector T cells rather than TFH cells in this response to acute bacterial infection.

AB - To investigate the importance of OX40 signals for physiological CD4(+) T-cell responses, an endogenous antigen-specific population of CD4(+) T cells that recognise the 2W1S peptide was assessed and temporal control of OX40 signals was achieved using blocking or agonistic antibodies (Abs) in vivo. Following infection with Listeria monocytogenes expressing 2W1S peptide, OX40 was briefly expressed by the responding 2W1S-specific CD4(+) T cells, but only on a subset that co-expressed effector cell markers. This population was specifically expanded by Ab-ligation of OX40 during priming, which also caused skewing of the memory response towards effector memory cells. Strikingly, this greatly enhanced effector response was accompanied by the loss of T follicular helper (TFH) cells and germinal centres. Mice deficient in OX40 and CD30 showed normal generation of TFH cells but impaired numbers of 2W1S-specific effector cells. OX40 was not expressed by 2W1S-specific memory cells, although it was rapidly up-regulated upon challenge whereupon Ab-ligation of OX40 specifically affected the effector subset. In summary, these data indicate that for CD4(+) T cells, OX40 signals are important for generation of effector T cells rather than TFH cells in this response to acute bacterial infection.

KW - CD4+ T cells

KW - Costimulation

KW - Germinal centre

KW - Memory

KW - OX40

U2 - 10.1002/eji.v44.8

DO - 10.1002/eji.v44.8

M3 - Article

C2 - 24771127

VL - 44

SP - 2437

EP - 2447

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 8

ER -