Overexpression of marA, soxS and acrB in veterinary isolates of Salmonella enterica rarely correlates with cyclohexane tolerance

Mark Webber, Antony Buckley, LP Randall, MJ Woodward, Laura Piddock

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Objectives: To determine the contribution of the AcrAB efflux system to cyclohexane tolerance in Salmonella enterica. Methods: The expression of the efflux pump gene, acrB, and regulators marA and soxS from 46 isolates of S. enterica of 14 different serovars was determined by comparative RT-PCR and denaturing HPLC analysis. Results: Twenty-one of the 46 isolates were cyclohexane tolerant, a phenotype associated with multiple antibiotic resistance (MAR) and overexpression of efflux pumps. Of the cyclohexane-tolerant isolates 81% were MAR, whereas only 44% of the cyclohexane-susceptible isolates were MAR, confirming the association between cyclohexane tolerance and MAR. However, there was no correlation between cyclohexane tolerance or MAR and overexpression of acrB, soxS or marA. Conclusions: These data suggest that cyclohexane tolerance in S. enterica can be mediated by an acrB-independent mechanism.
Original languageEnglish
Pages (from-to)673-9
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume57
Issue number4
Early online date21 Feb 2006
DOIs
Publication statusPublished - Apr 2006

Keywords

  • Animals
  • Animals, Domestic
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carrier Proteins
  • Cyclohexanes
  • DNA-Binding Proteins
  • Drug Resistance, Multiple, Bacterial
  • Escherichia coli Proteins
  • Membrane Transport Proteins
  • Microbial Sensitivity Tests
  • Salmonella enterica
  • Solvents
  • Trans-Activators

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