Abstract
Objectives: To determine the contribution of the AcrAB efflux system to cyclohexane tolerance in Salmonella enterica. Methods: The expression of the efflux pump gene, acrB, and regulators marA and soxS from 46 isolates of S. enterica of 14 different serovars was determined by comparative RT-PCR and denaturing HPLC analysis. Results: Twenty-one of the 46 isolates were cyclohexane tolerant, a phenotype associated with multiple antibiotic resistance (MAR) and overexpression of efflux pumps. Of the cyclohexane-tolerant isolates 81% were MAR, whereas only 44% of the cyclohexane-susceptible isolates were MAR, confirming the association between cyclohexane tolerance and MAR. However, there was no correlation between cyclohexane tolerance or MAR and overexpression of acrB, soxS or marA. Conclusions: These data suggest that cyclohexane tolerance in S. enterica can be mediated by an acrB-independent mechanism.
Original language | English |
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Pages (from-to) | 673-9 |
Number of pages | 7 |
Journal | Journal of Antimicrobial Chemotherapy |
Volume | 57 |
Issue number | 4 |
Early online date | 21 Feb 2006 |
DOIs | |
Publication status | Published - Apr 2006 |
Keywords
- Animals
- Animals, Domestic
- Anti-Bacterial Agents
- Bacterial Proteins
- Carrier Proteins
- Cyclohexanes
- DNA-Binding Proteins
- Drug Resistance, Multiple, Bacterial
- Escherichia coli Proteins
- Membrane Transport Proteins
- Microbial Sensitivity Tests
- Salmonella enterica
- Solvents
- Trans-Activators