Osteoblastic response to pectin nanocoating on titanium surfaces

Research output: Contribution to journalArticlepeer-review


  • Rikke Svava
  • Yu Yihua
  • Kenneth Brian Haugshøj
  • Kai Dirscherl
  • Steven B Levery
  • Inge Byg
  • Iben Damager
  • Martin W Nielsen
  • Bodil Jørgensen
  • Niklas Rye Jørgensen
  • Klaus Gotfredsen

Colleges, School and Institutes

External organisations

  • Microtechnology and Surface Analysis, Danish Technological Institute, Gregersensvej 8, 2630 Taastrup, Denmark.
  • Dansk Fundamental Metrologi A/S, Matematiktorvet 307, 2800 Lyngby, Denmark.
  • Copenhagen Center for Glycomics, Institute for Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
  • Department of Plant Environment Sciences, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
  • Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark.
  • Department of Systems Biology, Technical University of Denmark, Matematiktorvet, Building 301, Kgs. Lyngby DK-2800, Denmark.
  • Research Center for Ageing and Osteoporosis, Departments of Medicine and Diagnostics, Copenhagen University Hospital Glostrup, Ndr. Ringvej 57, 2600 Glostrup, Denmark.
  • Institute of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Nørre Allé 20, 2200 Copenhagen N, Denmark.
  • University of Copenhagen


Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro.


Original languageEnglish
Pages (from-to)117-25
Number of pages9
JournalMaterials Science and Engineering C
Early online date7 Jul 2014
Publication statusPublished - 1 Oct 2014


  • Carbohydrate Sequence, Cell Line, Tumor, Cell Proliferation, Coated Materials, Biocompatible, Humans, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Molecular Sequence Data, Nanostructures, Osteoblasts, Pectins, Photoelectron Spectroscopy, Titanium, Journal Article