Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds

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@article{6f6b6caf907c44bab3b486366f6c0453,
title = "Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds",
abstract = "We have developed an organotypic culture system that allows the production of bone tissue features on a centimeter scale. A composite, calcium phosphate–strained fibrin gel system is able to organize itself in the presence of osteoblastic cells, creating basic hierarchical units as seen in vivo, and can be modified to produce a range of other tissues that require such directional structuring. Constructs evolve over time into multi‐compositional structures containing a high mineral content and terminally differentiated, osteocyte‐like cells. These tissues can be cultured over extended durations (exceeding 1 year) and are responsive to a variety of chemical and biological agents. The platform can reduce the number of animals used in experimentation by acting as an intermediate stage in which more personalized research conditions can be generated. We provide a thorough description of the protocol used to successfully culture and modify this system, as well as guidance on compositional characterization.",
keywords = "biomaterials, bone, organotypic culture, osteocytes, self-organization",
author = "Alexandra Iordachescu and Richard Williams and Philippa Hulley and Liam Grover",
note = "(Citation) Iordachescu, A., Williams, R. L., Hulley, P. A., & Grover, L. M. (2019). Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds. Current Protocols in Stem Cell Biology, 48, e79. doi: 10.1002/cpsc.79",
year = "2019",
month = feb,
day = "1",
doi = "10.1002/cpsc.79",
language = "English",
volume = "48",
journal = "Current Protocols in Stem Cell Biology",
issn = "1941-7322",
publisher = "John Wiley & Sons",
number = "1",

}

RIS

TY - JOUR

T1 - Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds

AU - Iordachescu, Alexandra

AU - Williams, Richard

AU - Hulley, Philippa

AU - Grover, Liam

N1 - (Citation) Iordachescu, A., Williams, R. L., Hulley, P. A., & Grover, L. M. (2019). Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds. Current Protocols in Stem Cell Biology, 48, e79. doi: 10.1002/cpsc.79

PY - 2019/2/1

Y1 - 2019/2/1

N2 - We have developed an organotypic culture system that allows the production of bone tissue features on a centimeter scale. A composite, calcium phosphate–strained fibrin gel system is able to organize itself in the presence of osteoblastic cells, creating basic hierarchical units as seen in vivo, and can be modified to produce a range of other tissues that require such directional structuring. Constructs evolve over time into multi‐compositional structures containing a high mineral content and terminally differentiated, osteocyte‐like cells. These tissues can be cultured over extended durations (exceeding 1 year) and are responsive to a variety of chemical and biological agents. The platform can reduce the number of animals used in experimentation by acting as an intermediate stage in which more personalized research conditions can be generated. We provide a thorough description of the protocol used to successfully culture and modify this system, as well as guidance on compositional characterization.

AB - We have developed an organotypic culture system that allows the production of bone tissue features on a centimeter scale. A composite, calcium phosphate–strained fibrin gel system is able to organize itself in the presence of osteoblastic cells, creating basic hierarchical units as seen in vivo, and can be modified to produce a range of other tissues that require such directional structuring. Constructs evolve over time into multi‐compositional structures containing a high mineral content and terminally differentiated, osteocyte‐like cells. These tissues can be cultured over extended durations (exceeding 1 year) and are responsive to a variety of chemical and biological agents. The platform can reduce the number of animals used in experimentation by acting as an intermediate stage in which more personalized research conditions can be generated. We provide a thorough description of the protocol used to successfully culture and modify this system, as well as guidance on compositional characterization.

KW - biomaterials

KW - bone

KW - organotypic culture

KW - osteocytes

KW - self-organization

UR - http://www.scopus.com/inward/record.url?scp=85059955272&partnerID=8YFLogxK

U2 - 10.1002/cpsc.79

DO - 10.1002/cpsc.79

M3 - Article

VL - 48

JO - Current Protocols in Stem Cell Biology

JF - Current Protocols in Stem Cell Biology

SN - 1941-7322

IS - 1

M1 - e79

ER -