Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds

Research output: Contribution to journalArticlepeer-review

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  • University of Oxford


We have developed an organotypic culture system that allows the production of bone tissue features on a centimeter scale. A composite, calcium phosphate–strained fibrin gel system is able to organize itself in the presence of osteoblastic cells, creating basic hierarchical units as seen in vivo, and can be modified to produce a range of other tissues that require such directional structuring. Constructs evolve over time into multi‐compositional structures containing a high mineral content and terminally differentiated, osteocyte‐like cells. These tissues can be cultured over extended durations (exceeding 1 year) and are responsive to a variety of chemical and biological agents. The platform can reduce the number of animals used in experimentation by acting as an intermediate stage in which more personalized research conditions can be generated. We provide a thorough description of the protocol used to successfully culture and modify this system, as well as guidance on compositional characterization.

Bibliographic note

(Citation) Iordachescu, A., Williams, R. L., Hulley, P. A., & Grover, L. M. (2019). Organotypic culture of bone‐like structures using composite ceramic‐fibrin scaffolds. Current Protocols in Stem Cell Biology, 48, e79. doi: 10.1002/cpsc.79


Original languageEnglish
Article numbere79
JournalCurrent Protocols in Stem Cell Biology
Issue number1
Early online date14 Jan 2019
Publication statusPublished - 1 Feb 2019


  • biomaterials, bone, organotypic culture, osteocytes, self-organization

ASJC Scopus subject areas