Oral Epithelial Cell Culture Model for Studying the Pathogenesis of Chronic Inflammatory Disease

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Colleges, School and Institutes

Abstract

The interactions between bacteria, epithelium, and neutrophilic polymorphonuclear leukocytes (neutrophils) are the key to the initiation and progression of many chronic inflammatory-immune diseases. In addition, all can be influenced by external factors, such as micronutrients, thereby providing potentially novel approaches to therapy. This chapter will therefore provide detailed methods for core techniques involved in studying cellular and molecular epithelial responses to a bacterial challenge in relation to chronic inflammatory disease pathogenesis and therapy.

Details

Original languageEnglish
Title of host publicationOral biology:
Subtitle of host publicationmolecular techniques and applications
EditorsGregory J. Seymour, Mary P. Cullinan, Nicholas C. K. Heng
Publication statusPublished - 7 Dec 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1537
ISSN (Print)1064-3745

Keywords

  • Journal Article, Epithelium , Immunocytochemistry , Gene expression , Polymerase chain reaction , Electrophoresis , H400 oral epithelial cells , NF-kappa B , DNA , RNA