Optimisation of acquisition time in bioluminescence imaging

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Optimisation of acquisition time in bioluminescence imaging. / Taylor, Shelley L.; Mason, Suzannah K. G.; Glinton, Sophie; Cobbold, Mark; Styles, Iain B.; Dehghani, Hamid.

Proceedings of the SPIE. Vol. 9319 2015. p. 93191T-93191T-6.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Harvard

Taylor, SL, Mason, SKG, Glinton, S, Cobbold, M, Styles, IB & Dehghani, H 2015, Optimisation of acquisition time in bioluminescence imaging. in Proceedings of the SPIE. vol. 9319, pp. 93191T-93191T-6. https://doi.org/10.1117/12.2075661

APA

Taylor, S. L., Mason, S. K. G., Glinton, S., Cobbold, M., Styles, I. B., & Dehghani, H. (2015). Optimisation of acquisition time in bioluminescence imaging. In Proceedings of the SPIE (Vol. 9319, pp. 93191T-93191T-6) https://doi.org/10.1117/12.2075661

Vancouver

Taylor SL, Mason SKG, Glinton S, Cobbold M, Styles IB, Dehghani H. Optimisation of acquisition time in bioluminescence imaging. In Proceedings of the SPIE. Vol. 9319. 2015. p. 93191T-93191T-6 https://doi.org/10.1117/12.2075661

Author

Taylor, Shelley L. ; Mason, Suzannah K. G. ; Glinton, Sophie ; Cobbold, Mark ; Styles, Iain B. ; Dehghani, Hamid. / Optimisation of acquisition time in bioluminescence imaging. Proceedings of the SPIE. Vol. 9319 2015. pp. 93191T-93191T-6

Bibtex

@inproceedings{5648e7033c0e41b685bb23bfd2960439,
title = "Optimisation of acquisition time in bioluminescence imaging",
abstract = "Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.",
author = "Taylor, {Shelley L.} and Mason, {Suzannah K. G.} and Sophie Glinton and Mark Cobbold and Styles, {Iain B.} and Hamid Dehghani",
year = "2015",
doi = "10.1117/12.2075661",
language = "Undefined/Unknown",
volume = "9319",
pages = "93191T--93191T--6",
booktitle = "Proceedings of the SPIE",

}

RIS

TY - GEN

T1 - Optimisation of acquisition time in bioluminescence imaging

AU - Taylor, Shelley L.

AU - Mason, Suzannah K. G.

AU - Glinton, Sophie

AU - Cobbold, Mark

AU - Styles, Iain B.

AU - Dehghani, Hamid

PY - 2015

Y1 - 2015

N2 - Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.

AB - Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.

U2 - 10.1117/12.2075661

DO - 10.1117/12.2075661

M3 - Conference contribution

VL - 9319

SP - 93191T-93191T-6

BT - Proceedings of the SPIE

ER -