TY - JOUR
T1 - On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins.
AU - Sweet, Steve
AU - Cooper, Helen
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Electron capture dissociation (ECD) allows fragmentation of the phosphopeptide backbone while keeping the labile phospho-amino acid intact. This feature of ECD fragmentation, coupled with the acquisition of mass spectra at high mass accuracy, makes ECD well-suited to phosphorylation mapping. The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses.
AB - Electron capture dissociation (ECD) allows fragmentation of the phosphopeptide backbone while keeping the labile phospho-amino acid intact. This feature of ECD fragmentation, coupled with the acquisition of mass spectra at high mass accuracy, makes ECD well-suited to phosphorylation mapping. The following methods are designed to focus ECD events on phosphopeptides within a complex peptide sample, either by using phosphoric acid neutral loss peaks as a trigger or by targeted analysis of predetermined precursor masses.
U2 - 10.1007/978-1-60327-834-8_14
DO - 10.1007/978-1-60327-834-8_14
M3 - Article
C2 - 19241014
SN - 1940-6029
VL - 527
SP - 191
EP - 199
JO - Methods in molecular biology
JF - Methods in molecular biology
ER -