Oncostatin M and Kit-Ligand control hematopoietic stem cell fate during zebrafish embryogenesis

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

External organisations

  • University of Geneva

Abstract

Understanding the molecular pathways controlling hematopoietic stem cell specification and expansion is a necessary milestone to perform regenerative medicine. Here, we used the zebrafish model to study the role of the ckit signaling pathway in this process. We show the importance of kitb/kitlgb signaling in the specification and expansion of hematopoietic stem cells (HSCs), in the hemogenic endothelium and caudal hematopoietic tissue (CHT), respectively. Moreover, we identified the zebrafish ortholog of Oncostatin M (osm) in the zebrafish genome. We show that the osm/osmr pathway acts upstream of kitb during specification of the hemogenic endothelium, while both pathways act synergistically to expand HSCs in the CHT. Moreover, we found that osm, in addition to its role in promoting HSC proliferation, inhibits HSC commitment to the lymphoid fate. Altogether, our data identified two cytokines, kitlgb and osm, secreted by the vascular niche, that control HSCs during early embryonic development.

Details

Original languageEnglish
Pages (from-to)1920-1934
JournalStem Cell Reports
Volume10
Issue number6
Early online date17 May 2018
Publication statusPublished - 5 Jun 2018

Keywords

  • hematopoietic stem cells, zebrafish, oncostatin M, kit-ligand, hematopoietic niche, oncostatin M receptor (osmr), cKIT