Abstract
Objectives: Nupharidine (6,6′‐Dihydroxythiobinupharidine), purified from the aquatic plant Nuphar lutea leaves (Water lily) prompts antimicrobial activity of immune cells. The aim of the study was to test the effect of Nupharidine on neutrophil function against Aggregatibacter actinomycetemcomitans, JP2 clone (Aa‐JP2).
Methods: Neutrophils derived from the human cell line HL60 and human peripheral blood derived from aggressive periodontitis and periodontally healthy subjects were incubated with Nupharidine or vehicle and inoculated with JP2. Bacterial survival was tested using viable counts on blood agar (CFU's). Neutrophils’ necrosis/apoptosis, reactive oxygen species (ROS) production, phagocytosis and neutrophil extracellular traps (NET) production following infection were tested, as well as markers of neutrophil priming.
Results: Nupharidine had no direct bactericidal effect on JP2, but it enhanced Aa‐JP2 clearance by neutrophils. Nupharidine enhanced neutrophil phagocytosis, ROS production and NET formation during JP2 infection. Furthermore, Nupharidine enhanced the expression of certain markers of neutrophils priming, specifically iCAM1, DECTIN‐2 and intracellular IL‐1β.
Conclusion: Nupharidine was shown to promote neutrophil effector bactericidal functions, boosting Aa‐JP2 clearance. The results point to the potential of Nupharidine as an adjunctive agent in the treatment of Aa‐JP2 periodontitis, but this should be tested initially using pre‐clinical and clinical studies.
Methods: Neutrophils derived from the human cell line HL60 and human peripheral blood derived from aggressive periodontitis and periodontally healthy subjects were incubated with Nupharidine or vehicle and inoculated with JP2. Bacterial survival was tested using viable counts on blood agar (CFU's). Neutrophils’ necrosis/apoptosis, reactive oxygen species (ROS) production, phagocytosis and neutrophil extracellular traps (NET) production following infection were tested, as well as markers of neutrophil priming.
Results: Nupharidine had no direct bactericidal effect on JP2, but it enhanced Aa‐JP2 clearance by neutrophils. Nupharidine enhanced neutrophil phagocytosis, ROS production and NET formation during JP2 infection. Furthermore, Nupharidine enhanced the expression of certain markers of neutrophils priming, specifically iCAM1, DECTIN‐2 and intracellular IL‐1β.
Conclusion: Nupharidine was shown to promote neutrophil effector bactericidal functions, boosting Aa‐JP2 clearance. The results point to the potential of Nupharidine as an adjunctive agent in the treatment of Aa‐JP2 periodontitis, but this should be tested initially using pre‐clinical and clinical studies.
| Original language | English |
|---|---|
| Pages (from-to) | 62-71 |
| Number of pages | 10 |
| Journal | Journal of Clinical Periodontology |
| Volume | 46 |
| Issue number | 1 |
| Early online date | 29 Oct 2018 |
| DOIs | |
| Publication status | Published - Jan 2019 |
Access to Document
- Dan_Henry_Levy_et_al_Nupharidine_enhances_aggregatibacter_actinomycetemcomitans_Journal_of_Clinical_Periodontology_2018
Checked for eligibility: 25/10/2018 This is the pre-peer reviewed version of the following article: Levy DH, Chapple ILC, Shapira L, Golan‐Goldhirsh A, Gopas J, Polak D. Nupharidine enhances Aggregatibacter actinomycetemcomitans clearance by priming neutrophils and augmenting their effector functions. J Clin Periodontol. 2018;00:1–10., which has been published in final form at: https://doi.org/10.1111/jcpe.13036. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.