Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage

Research output: Contribution to journalArticlepeer-review

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Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage. / Grzechnik, Pawel; Szczepaniak, Sylwia A; Dhir, Somdutta; Pastucha, Anna; Parslow, Hannah; Matuszek, Zaneta; Mischo, Hannah E; Kufel, Joanna; Proudfoot, Nicholas J.

In: Nature Communications, Vol. 9, 1783, 03.05.2018.

Research output: Contribution to journalArticlepeer-review

Harvard

Grzechnik, P, Szczepaniak, SA, Dhir, S, Pastucha, A, Parslow, H, Matuszek, Z, Mischo, HE, Kufel, J & Proudfoot, NJ 2018, 'Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage', Nature Communications, vol. 9, 1783. https://doi.org/10.1038/s41467-018-04094-y

APA

Grzechnik, P., Szczepaniak, S. A., Dhir, S., Pastucha, A., Parslow, H., Matuszek, Z., Mischo, H. E., Kufel, J., & Proudfoot, N. J. (2018). Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage. Nature Communications, 9, [1783]. https://doi.org/10.1038/s41467-018-04094-y

Vancouver

Author

Grzechnik, Pawel ; Szczepaniak, Sylwia A ; Dhir, Somdutta ; Pastucha, Anna ; Parslow, Hannah ; Matuszek, Zaneta ; Mischo, Hannah E ; Kufel, Joanna ; Proudfoot, Nicholas J. / Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage. In: Nature Communications. 2018 ; Vol. 9.

Bibtex

@article{4cf2de24b8c04121975f8902d4091e58,
title = "Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage",
abstract = "Small nucleolar RNA (snoRNA) are conserved and essential non-coding RNA that are transcribed by RNA Polymerase II (Pol II). Two snoRNA classes, formerly distinguished by their structure and ribonucleoprotein composition, act as guide RNA to target RNA such as ribosomal RNA, and thereby introduce specific modifications. We have studied the 5'end processing of individually transcribed snoRNA in S. cerevisiae to define their role in snoRNA biogenesis and functionality. Here we show that pre-snoRNA processing by the endonuclease Rnt1 occurs co-transcriptionally with removal of the m7G cap facilitating the formation of box C/D snoRNA. Failure of this process causes aberrant 3'end processing and mislocalization of snoRNA to the cytoplasm. Consequently, Rnt1-dependent 5'end processing of box C/D snoRNA is critical for snoRNA-dependent methylation of ribosomal RNA. Our results reveal that the 5'end processing of box C/D snoRNA defines their distinct pathway of maturation.",
keywords = "RNA, RNA metabolism",
author = "Pawel Grzechnik and Szczepaniak, {Sylwia A} and Somdutta Dhir and Anna Pastucha and Hannah Parslow and Zaneta Matuszek and Mischo, {Hannah E} and Joanna Kufel and Proudfoot, {Nicholas J}",
year = "2018",
month = may,
day = "3",
doi = "10.1038/s41467-018-04094-y",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage

AU - Grzechnik, Pawel

AU - Szczepaniak, Sylwia A

AU - Dhir, Somdutta

AU - Pastucha, Anna

AU - Parslow, Hannah

AU - Matuszek, Zaneta

AU - Mischo, Hannah E

AU - Kufel, Joanna

AU - Proudfoot, Nicholas J

PY - 2018/5/3

Y1 - 2018/5/3

N2 - Small nucleolar RNA (snoRNA) are conserved and essential non-coding RNA that are transcribed by RNA Polymerase II (Pol II). Two snoRNA classes, formerly distinguished by their structure and ribonucleoprotein composition, act as guide RNA to target RNA such as ribosomal RNA, and thereby introduce specific modifications. We have studied the 5'end processing of individually transcribed snoRNA in S. cerevisiae to define their role in snoRNA biogenesis and functionality. Here we show that pre-snoRNA processing by the endonuclease Rnt1 occurs co-transcriptionally with removal of the m7G cap facilitating the formation of box C/D snoRNA. Failure of this process causes aberrant 3'end processing and mislocalization of snoRNA to the cytoplasm. Consequently, Rnt1-dependent 5'end processing of box C/D snoRNA is critical for snoRNA-dependent methylation of ribosomal RNA. Our results reveal that the 5'end processing of box C/D snoRNA defines their distinct pathway of maturation.

AB - Small nucleolar RNA (snoRNA) are conserved and essential non-coding RNA that are transcribed by RNA Polymerase II (Pol II). Two snoRNA classes, formerly distinguished by their structure and ribonucleoprotein composition, act as guide RNA to target RNA such as ribosomal RNA, and thereby introduce specific modifications. We have studied the 5'end processing of individually transcribed snoRNA in S. cerevisiae to define their role in snoRNA biogenesis and functionality. Here we show that pre-snoRNA processing by the endonuclease Rnt1 occurs co-transcriptionally with removal of the m7G cap facilitating the formation of box C/D snoRNA. Failure of this process causes aberrant 3'end processing and mislocalization of snoRNA to the cytoplasm. Consequently, Rnt1-dependent 5'end processing of box C/D snoRNA is critical for snoRNA-dependent methylation of ribosomal RNA. Our results reveal that the 5'end processing of box C/D snoRNA defines their distinct pathway of maturation.

KW - RNA

KW - RNA metabolism

U2 - 10.1038/s41467-018-04094-y

DO - 10.1038/s41467-018-04094-y

M3 - Article

C2 - 29725044

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 1783

ER -