Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

Jing Zhang; Zhihua Wang; Yong Jiang; Zhongying Niu; Lei Fu; Zhirong Luo; Paul Cooper; Anthony Smith; Wenxi He

doi:10.1016/j.yexcr.2015.01.020

Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

Jing Zhang, Zhihua Wang, Yong Jiang, Zhongying Niu, Lei Fu, Zhirong Luo, Paul Cooper, Anthony Smith, Wenxi He

Dentistry

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Abstract

The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs.

Original language	English
Pages (from-to)	259-266
Journal	Experimental Cell Research
Volume	332
Issue number	2
Early online date	7 Feb 2015
DOIs	https://doi.org/10.1016/j.yexcr.2015.01.020
Publication status	Published - 15 Mar 2015

Keywords

SCAPs
Nuclear factor
Proliferation
Differentiation

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10.1016/j.yexcr.2015.01.020

Zhang_et_al_Nuclear_factor_I_C_Experimental_Cell_Research_2015
NOTICE: this is the author’s version of a work that was accepted for publication. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published as Jing Zhang, Zhihua Wang, Jiang Yong, Zhongying Niu, Lei Fu, Zhirong Luo, Paul R. Cooper, Anthony J. Smith,Wenxi He, Nuclear factor I-C promotes proliferation and differentiation of apical papilla-derived Human stem cells in vitro, Experimental Cell Research, http://dx.doi.org/10.1016/j. yexcr.2015.01.020
Accepted author manuscript, 764 KBLicence: Other (please specify with Rights Statement)

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title = "Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro",

abstract = "The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs.",

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author = "Jing Zhang and Zhihua Wang and Yong Jiang and Zhongying Niu and Lei Fu and Zhirong Luo and Paul Cooper and Anthony Smith and Wenxi He",

year = "2015",

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doi = "10.1016/j.yexcr.2015.01.020",

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T1 - Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

AU - Zhang, Jing

AU - Wang, Zhihua

AU - Jiang, Yong

AU - Niu, Zhongying

AU - Fu, Lei

AU - Luo, Zhirong

AU - Cooper, Paul

AU - Smith, Anthony

AU - He, Wenxi

PY - 2015/3/15

Y1 - 2015/3/15

N2 - The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs.

AB - The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs.

KW - SCAPs

KW - Nuclear factor

KW - Proliferation

KW - Differentiation

U2 - 10.1016/j.yexcr.2015.01.020

DO - 10.1016/j.yexcr.2015.01.020

M3 - Article

SN - 0014-4827

VL - 332

SP - 259

EP - 266

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

Abstract

Keywords

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