Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

Research output: Contribution to journalArticlepeer-review


  • Jing Zhang
  • Zhihua Wang
  • Yong Jiang
  • Zhongying Niu
  • Lei Fu
  • Zhirong Luo
  • Wenxi He

Colleges, School and Institutes


The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs.


Original languageEnglish
Pages (from-to)259-266
JournalExperimental Cell Research
Issue number2
Early online date7 Feb 2015
Publication statusPublished - 15 Mar 2015


  • SCAPs, Nuclear factor, Proliferation, Differentiation