Norovirus translation requires an interaction between the C terminus of the genome-linked viral protein VPg and eukaryotic translation initiation factor 4G

Research output: Contribution to journalArticle

Authors

  • L. Chung
  • E. N. Leen
  • E. P. Emmott
  • Y. Chaudhry
  • L. O. Roberts
  • S. Curry
  • N. Locker
  • I. G. Goodfellow

Colleges, School and Institutes

Abstract

Viruses have evolved a variety of mechanisms to usurp the host cell translation machinery to enable translation of the viral genome in the presence of high levels of cellular mRNAs. Noroviruses, a major cause of gastroenteritis in man, have evolved a mechanism that relies on the interaction of translation initiation factors with the virus-encoded VPg protein covalently linked to the 5′ end of the viral RNA. To further characterize this novel mechanism of translation initiation, we have used proteomics to identify the components of the norovirus translation initiation factor complex. This approach revealed that VPg binds directly to the eIF4F complex, with a high affinity interaction occurring between VPg and eIF4G. Mutational analyses indicated that the C-terminal region of VPg is important for the VPg-eIF4G interaction; viruses with mutations that alter or disrupt this interaction are debilitated or non-viable. Our results shed new light on the unusual mechanisms of protein-directed translation initiation.

Details

Original languageEnglish
Pages (from-to)21738-21750
JournalJournal of Biological Chemistry
Volume289
Issue number31
Early online date14 Jun 2014
Publication statusPublished - 1 Aug 2014

Keywords

  • Eukaryotic Translation Initiation Factor 4E (eIF4E), Plus-stranded RNA Virus, Translation, Translation Initiation Factor, Virus, VPg, eIF4G, Norovirus