Nonredundant roles of platelet glycoprotein VI and integrin αIIbβ3 in fibrin-mediated microthrombus formation

Research output: Contribution to journalArticlepeer-review

Authors

  • Gina Perrella
  • Jingnan Huang
  • Isabella Provenzale
  • Frauke Swieringa
  • Floor Heubel-Moenen
  • Richard W Farndale
  • Mark Roest
  • Paola Van Der Meijden
  • Robert A. S. Ariëns
  • Martine Jandrot-Perrus
  • Johan W M Heemskerk

Abstract

Objective: Fibrin is considered to strengthen thrombus formation via integrin αIIbβ3, but recent findings indicate that fibrin can also act as ligand for platelet glycoprotein VI.

Approach and Results: To investigate the thrombus-forming potential of fibrin and the roles of platelet receptors herein, we generated a range of immobilized fibrin surfaces, some of which were cross-linked with factor XIIIa and contained VWF-BP (von Willebrand factor-binding peptide). Multicolor microfluidics assays with whole-blood flowed at high shear rate (1000 s−1) indicated that the fibrin surfaces, regardless of the presence of factor XIIIa or VWF-BP, supported platelet adhesion and activation (P-selectin expression), but only microthrombi were formed consisting of bilayers of platelets. Fibrinogen surfaces produced similar microthrombi. Markedly, tiggering of coagulation with tissue factor or blocking of thrombin no more than moderately affected the fibrin-induced microthrombus formation. Absence of αIIbβ3 in Glanzmann thrombasthenia annulled platelet adhesion. Blocking of glycoprotein VI with Fab 9O12 substantially, but incompletely reduced platelet secretion, Ca2+ signaling and aggregation, while inhibition of Syk further reduced these responses. In platelet suspension, glycoprotein VI blockage or Syk inhibition prevented fibrin-induced platelet aggregation. Microthrombi on fibrin surfaces triggered only minimal thrombin generation, in spite of thrombin binding to the fibrin fibers.

Conclusions: Together, these results indicate that fibrin fibers, regardless of their way of formation, act as a consolidating surface in microthrombus formation via nonredundant roles of platelet glycoprotein VI and integrin αIIbβ3 through signaling via Syk and low-level Ca2+ rises.

Bibliographic note

Sources of Funding: G. Perrella is supported by a joint PhD scholarship of Maastricht and Birmingham Universities. J. Huang and I. Provenzale are supported by the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 766118, and are registered in the PhD programs of Maastricht and Santiago da Compostela Universities (J. Huang) or Maastricht and Reading Universities (I. Provenzale). S.P. Watson and R.A.S. Ariëns are supported by a Wellcome Trust Joint Investigator Award (204951/Z/16/Z). S.P. Watson holds a British Heart Foundation Chair (CH/03/003). Copyright: © 2020 American Heart Association, Inc.

Details

Original languageEnglish
Pages (from-to)e97–e111
Number of pages15
JournalArteriosclerosis Thrombosis and Vascular Biology
Volume41
Issue number2
Early online date3 Dec 2020
Publication statusPublished - Feb 2021

Keywords

  • fibrin, thrombin, platelet aggregation, blood platelet, microfluidics