Non-invasive assessment of glioma microstructure using VERDICT MRI: correlation with histology

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Non-invasive assessment of glioma microstructure using VERDICT MRI : correlation with histology. / Zaccagna, Fulvio; Riemer, Frank; Priest, Andrew N; McLean, Mary A; Allinson, Kieren; Grist, James T; Dragos, Carmen; Matys, Tomasz; Gillard, Jonathan H; Watts, Colin; Price, Stephen J; Graves, Martin J; Gallagher, Ferdia A.

In: European Radiology, Vol. 29, No. 10, 01.10.2019, p. 5559-5566.

Research output: Contribution to journalArticle

Harvard

Zaccagna, F, Riemer, F, Priest, AN, McLean, MA, Allinson, K, Grist, JT, Dragos, C, Matys, T, Gillard, JH, Watts, C, Price, SJ, Graves, MJ & Gallagher, FA 2019, 'Non-invasive assessment of glioma microstructure using VERDICT MRI: correlation with histology', European Radiology, vol. 29, no. 10, pp. 5559-5566. https://doi.org/10.1007/s00330-019-6011-8

APA

Zaccagna, F., Riemer, F., Priest, A. N., McLean, M. A., Allinson, K., Grist, J. T., Dragos, C., Matys, T., Gillard, J. H., Watts, C., Price, S. J., Graves, M. J., & Gallagher, F. A. (2019). Non-invasive assessment of glioma microstructure using VERDICT MRI: correlation with histology. European Radiology, 29(10), 5559-5566. https://doi.org/10.1007/s00330-019-6011-8

Vancouver

Zaccagna F, Riemer F, Priest AN, McLean MA, Allinson K, Grist JT et al. Non-invasive assessment of glioma microstructure using VERDICT MRI: correlation with histology. European Radiology. 2019 Oct 1;29(10):5559-5566. https://doi.org/10.1007/s00330-019-6011-8

Author

Zaccagna, Fulvio ; Riemer, Frank ; Priest, Andrew N ; McLean, Mary A ; Allinson, Kieren ; Grist, James T ; Dragos, Carmen ; Matys, Tomasz ; Gillard, Jonathan H ; Watts, Colin ; Price, Stephen J ; Graves, Martin J ; Gallagher, Ferdia A. / Non-invasive assessment of glioma microstructure using VERDICT MRI : correlation with histology. In: European Radiology. 2019 ; Vol. 29, No. 10. pp. 5559-5566.

Bibtex

@article{b0771bb392884aceb5e11b211135407d,
title = "Non-invasive assessment of glioma microstructure using VERDICT MRI: correlation with histology",
abstract = "PURPOSE: This prospective study evaluated the use of vascular, extracellular and restricted diffusion for cytometry in tumours (VERDICT) MRI to investigate the tissue microstructure in glioma. VERDICT-derived parameters were correlated with both histological features and tumour subtype and were also used to explore the peritumoural region.METHODS: Fourteen consecutive treatment-na{\"i}ve patients (43.5 years ± 15.1 years, six males, eight females) with suspected glioma underwent diffusion-weighted imaging including VERDICT modelling. Tumour cell radius and intracellular and combined extracellular/vascular volumes were estimated using a framework based on linearisation and convex optimisation. An experienced neuroradiologist outlined the peritumoural oedema, enhancing tumour and necrosis on T2-weighted imaging and contrast-enhanced T1-weighted imaging. The same regions of interest were applied to the co-registered VERDICT maps to calculate the microstructure parameters. Pathology sections were analysed with semi-automated software to measure cellularity and cell size.RESULTS: VERDICT parameters were successfully calculated in all patients. The imaging-derived results showed a larger intracellular volume fraction in high-grade glioma compared to low-grade glioma (0.13 ± 0.07 vs. 0.08 ± 0.02, respectively; p = 0.05) and a trend towards a smaller extracellular/vascular volume fraction (0.88 ± 0.07 vs. 0.92 ± 0.04, respectively; p = 0.10). The conventional apparent diffusion coefficient was higher in low-grade gliomas compared to high-grade gliomas, but this difference was not statistically significant (1.22 ± 0.13 × 10-3 mm2/s vs. 0.98 ± 0.38 × 10-3 mm2/s, respectively; p = 0.18).CONCLUSION: This feasibility study demonstrated that VERDICT MRI can be used to explore the tissue microstructure of glioma using an abbreviated protocol. The VERDICT parameters of tissue structure correlated with those derived on histology. The method shows promise as a potential test for diagnostic stratification and treatment response monitoring in the future.KEY POINTS: • VERDICT MRI is an advanced diffusion technique which has been correlated with histopathological findings obtained at surgery from patients with glioma in this study. • The intracellular volume fraction measured with VERDICT was larger in high-grade tumours compared to that in low-grade tumours. • The results were complementary to measurements from conventional diffusion-weighted imaging, and the technique could be performed in a clinically feasible timescale.",
keywords = "Brain neoplasms, Cancer, Diagnostic imaging, Diffusion magnetic resonance imaging, Glioma",
author = "Fulvio Zaccagna and Frank Riemer and Priest, {Andrew N} and McLean, {Mary A} and Kieren Allinson and Grist, {James T} and Carmen Dragos and Tomasz Matys and Gillard, {Jonathan H} and Colin Watts and Price, {Stephen J} and Graves, {Martin J} and Gallagher, {Ferdia A}",
year = "2019",
month = oct,
day = "1",
doi = "10.1007/s00330-019-6011-8",
language = "English",
volume = "29",
pages = "5559--5566",
journal = "European Radiology",
issn = "0938-7994",
publisher = "Springer",
number = "10",

}

RIS

TY - JOUR

T1 - Non-invasive assessment of glioma microstructure using VERDICT MRI

T2 - correlation with histology

AU - Zaccagna, Fulvio

AU - Riemer, Frank

AU - Priest, Andrew N

AU - McLean, Mary A

AU - Allinson, Kieren

AU - Grist, James T

AU - Dragos, Carmen

AU - Matys, Tomasz

AU - Gillard, Jonathan H

AU - Watts, Colin

AU - Price, Stephen J

AU - Graves, Martin J

AU - Gallagher, Ferdia A

PY - 2019/10/1

Y1 - 2019/10/1

N2 - PURPOSE: This prospective study evaluated the use of vascular, extracellular and restricted diffusion for cytometry in tumours (VERDICT) MRI to investigate the tissue microstructure in glioma. VERDICT-derived parameters were correlated with both histological features and tumour subtype and were also used to explore the peritumoural region.METHODS: Fourteen consecutive treatment-naïve patients (43.5 years ± 15.1 years, six males, eight females) with suspected glioma underwent diffusion-weighted imaging including VERDICT modelling. Tumour cell radius and intracellular and combined extracellular/vascular volumes were estimated using a framework based on linearisation and convex optimisation. An experienced neuroradiologist outlined the peritumoural oedema, enhancing tumour and necrosis on T2-weighted imaging and contrast-enhanced T1-weighted imaging. The same regions of interest were applied to the co-registered VERDICT maps to calculate the microstructure parameters. Pathology sections were analysed with semi-automated software to measure cellularity and cell size.RESULTS: VERDICT parameters were successfully calculated in all patients. The imaging-derived results showed a larger intracellular volume fraction in high-grade glioma compared to low-grade glioma (0.13 ± 0.07 vs. 0.08 ± 0.02, respectively; p = 0.05) and a trend towards a smaller extracellular/vascular volume fraction (0.88 ± 0.07 vs. 0.92 ± 0.04, respectively; p = 0.10). The conventional apparent diffusion coefficient was higher in low-grade gliomas compared to high-grade gliomas, but this difference was not statistically significant (1.22 ± 0.13 × 10-3 mm2/s vs. 0.98 ± 0.38 × 10-3 mm2/s, respectively; p = 0.18).CONCLUSION: This feasibility study demonstrated that VERDICT MRI can be used to explore the tissue microstructure of glioma using an abbreviated protocol. The VERDICT parameters of tissue structure correlated with those derived on histology. The method shows promise as a potential test for diagnostic stratification and treatment response monitoring in the future.KEY POINTS: • VERDICT MRI is an advanced diffusion technique which has been correlated with histopathological findings obtained at surgery from patients with glioma in this study. • The intracellular volume fraction measured with VERDICT was larger in high-grade tumours compared to that in low-grade tumours. • The results were complementary to measurements from conventional diffusion-weighted imaging, and the technique could be performed in a clinically feasible timescale.

AB - PURPOSE: This prospective study evaluated the use of vascular, extracellular and restricted diffusion for cytometry in tumours (VERDICT) MRI to investigate the tissue microstructure in glioma. VERDICT-derived parameters were correlated with both histological features and tumour subtype and were also used to explore the peritumoural region.METHODS: Fourteen consecutive treatment-naïve patients (43.5 years ± 15.1 years, six males, eight females) with suspected glioma underwent diffusion-weighted imaging including VERDICT modelling. Tumour cell radius and intracellular and combined extracellular/vascular volumes were estimated using a framework based on linearisation and convex optimisation. An experienced neuroradiologist outlined the peritumoural oedema, enhancing tumour and necrosis on T2-weighted imaging and contrast-enhanced T1-weighted imaging. The same regions of interest were applied to the co-registered VERDICT maps to calculate the microstructure parameters. Pathology sections were analysed with semi-automated software to measure cellularity and cell size.RESULTS: VERDICT parameters were successfully calculated in all patients. The imaging-derived results showed a larger intracellular volume fraction in high-grade glioma compared to low-grade glioma (0.13 ± 0.07 vs. 0.08 ± 0.02, respectively; p = 0.05) and a trend towards a smaller extracellular/vascular volume fraction (0.88 ± 0.07 vs. 0.92 ± 0.04, respectively; p = 0.10). The conventional apparent diffusion coefficient was higher in low-grade gliomas compared to high-grade gliomas, but this difference was not statistically significant (1.22 ± 0.13 × 10-3 mm2/s vs. 0.98 ± 0.38 × 10-3 mm2/s, respectively; p = 0.18).CONCLUSION: This feasibility study demonstrated that VERDICT MRI can be used to explore the tissue microstructure of glioma using an abbreviated protocol. The VERDICT parameters of tissue structure correlated with those derived on histology. The method shows promise as a potential test for diagnostic stratification and treatment response monitoring in the future.KEY POINTS: • VERDICT MRI is an advanced diffusion technique which has been correlated with histopathological findings obtained at surgery from patients with glioma in this study. • The intracellular volume fraction measured with VERDICT was larger in high-grade tumours compared to that in low-grade tumours. • The results were complementary to measurements from conventional diffusion-weighted imaging, and the technique could be performed in a clinically feasible timescale.

KW - Brain neoplasms

KW - Cancer

KW - Diagnostic imaging

KW - Diffusion magnetic resonance imaging

KW - Glioma

UR - http://www.scopus.com/inward/record.url?scp=85063208065&partnerID=8YFLogxK

U2 - 10.1007/s00330-019-6011-8

DO - 10.1007/s00330-019-6011-8

M3 - Article

C2 - 30888488

VL - 29

SP - 5559

EP - 5566

JO - European Radiology

JF - European Radiology

SN - 0938-7994

IS - 10

ER -