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Abstract
The mechanisms of T cell help for production of antilipid antibodies are largely unknown. This study shows that invariant Nk T cells (INK T cells) and B cells cooperate in a model of antilipid antigen-specific antibody responses. We use a model haptenated lipid molecule, 4-hydroxy-3-nitrophenyl-alpha GalactosylCeramide (NP-alpha GalCer), to demonstrate that INK T cells provide cognate help to lipid-antigen-presenting B cells. B cells proliferate and IgG anti-NP is produced from in vivo-immunized mice and in vitro cocultures of B and iNK T cells after exposure to NP-alpha GalCer, but not closely related control glycolipids. This B cell response is absent in CD1d(-/-) and J alpha 18(-/-) mice but not CD4(-/-) mice. The antibody response to NP-alpha GalCer is dominated by the IgM, IgG3, and IgG2c isotypes, and marginal zone B cells stimulate better in vitro lipid antigen-driven proliferation than follicular B cells, suggesting an important role for this B cell subset. INK T cell help for 8 cells is shown to involve cognate help from CD1d-instructed lipid-specific INK T cells, with help provided via CD40L, B7-1/B7-2, and IFN-gamma, but not IL-4. This model provides evidence of INK T cell help for antilipid antibody production, an important aspect of infections, autoimmune diseases, and vaccine development. Our findings also now allow prediction of those microbial antigens that would be expected to elicit cognate iNKT cell help for antibody production, namely those that can stimulate iNKT cells and at the same time have a polar moiety that can be recognized by antibodies.
Original language | English |
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Pages (from-to) | 8339-8344 |
Number of pages | 6 |
Journal | National Academy of Sciences. Proceedings |
Volume | 105 |
Issue number | 24 |
DOIs | |
Publication status | Published - 1 Jun 2008 |
Keywords
- alpha-galactosylceramide
- T cell helper
- antibodies
- iNK T cell
- CD1d
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Dive into the research topics of 'NK T cells provide lipid antigen-specific cognate help for B cells'. Together they form a unique fingerprint.Projects
- 1 Finished
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Modulation of Immune Responses by aGalCer Analogues Through Differential Activation of CD1d-restricted NKT Cells
Besra, D. & Lammas, T.
1/06/05 → 30/11/09
Project: Research Councils