New twists on detecting mRNA modification dynamics

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New twists on detecting mRNA modification dynamics. / Anreiter, Ina ; Mir, Quoseena; Simpson, Jared ; Janga , Sarath Chandra ; Soller, Matthias.

In: Trends in Biotechnology, 30.06.2020.

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Anreiter, Ina ; Mir, Quoseena ; Simpson, Jared ; Janga , Sarath Chandra ; Soller, Matthias. / New twists on detecting mRNA modification dynamics. In: Trends in Biotechnology. 2020.

Bibtex

@article{75cd571aa4224b0b8638362dd9941df4,
title = "New twists on detecting mRNA modification dynamics",
abstract = "Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.",
keywords = "Nanopore, mRNA modifications, m6A, m6Am, m5C, 2'-O-ribose methylation",
author = "Ina Anreiter and Quoseena Mir and Jared Simpson and Janga, {Sarath Chandra} and Matthias Soller",
year = "2020",
month = jun,
day = "30",
doi = "10.1016/j.tibtech.2020.06.002",
language = "English",
journal = "Trends in Biotechnology",
issn = "0167-7799",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - New twists on detecting mRNA modification dynamics

AU - Anreiter, Ina

AU - Mir, Quoseena

AU - Simpson, Jared

AU - Janga , Sarath Chandra

AU - Soller, Matthias

PY - 2020/6/30

Y1 - 2020/6/30

N2 - Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

AB - Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

KW - Nanopore

KW - mRNA modifications

KW - m6A

KW - m6Am

KW - m5C

KW - 2'-O-ribose methylation

U2 - 10.1016/j.tibtech.2020.06.002

DO - 10.1016/j.tibtech.2020.06.002

M3 - Review article

JO - Trends in Biotechnology

JF - Trends in Biotechnology

SN - 0167-7799

ER -