New twists on detecting mRNA modification dynamics
Research output: Contribution to journal › Review article › peer-review
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New twists on detecting mRNA modification dynamics. / Anreiter, Ina ; Mir, Quoseena; Simpson, Jared ; Janga , Sarath Chandra ; Soller, Matthias.
In: Trends in Biotechnology, 30.06.2020.Research output: Contribution to journal › Review article › peer-review
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TY - JOUR
T1 - New twists on detecting mRNA modification dynamics
AU - Anreiter, Ina
AU - Mir, Quoseena
AU - Simpson, Jared
AU - Janga , Sarath Chandra
AU - Soller, Matthias
PY - 2020/6/30
Y1 - 2020/6/30
N2 - Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.
AB - Modified nucleotides in messenger RNA (mRNA) are an essential addition to the standard genetic code of four nucleotides in animals, plants and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundancy of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances are required to increase confidence. Single molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations and we will summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.
KW - Nanopore
KW - mRNA modifications
KW - m6A
KW - m6Am
KW - m5C
KW - 2'-O-ribose methylation
U2 - 10.1016/j.tibtech.2020.06.002
DO - 10.1016/j.tibtech.2020.06.002
M3 - Review article
JO - Trends in Biotechnology
JF - Trends in Biotechnology
SN - 0167-7799
ER -