New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen

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New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen. / Wang, Ludi; Triviño, Marina; Lin, Zongcheng; Carli, José; Eaves, Deborah J; Van Damme, Daniёl; Nowack, Moritz K; Franklin-Tong, Vernonica E; Bosch, Maurice.

In: Journal of Experimental Botany, Vol. 71, No. 8, 23.04.2020, p. 2451-2463.

Research output: Contribution to journalArticlepeer-review

Harvard

Wang, L, Triviño, M, Lin, Z, Carli, J, Eaves, DJ, Van Damme, D, Nowack, MK, Franklin-Tong, VE & Bosch, M 2020, 'New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen', Journal of Experimental Botany, vol. 71, no. 8, pp. 2451-2463. https://doi.org/10.1093/jxb/eraa092

APA

Wang, L., Triviño, M., Lin, Z., Carli, J., Eaves, D. J., Van Damme, D., Nowack, M. K., Franklin-Tong, V. E., & Bosch, M. (2020). New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen. Journal of Experimental Botany, 71(8), 2451-2463. https://doi.org/10.1093/jxb/eraa092

Vancouver

Author

Wang, Ludi ; Triviño, Marina ; Lin, Zongcheng ; Carli, José ; Eaves, Deborah J ; Van Damme, Daniёl ; Nowack, Moritz K ; Franklin-Tong, Vernonica E ; Bosch, Maurice. / New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen. In: Journal of Experimental Botany. 2020 ; Vol. 71, No. 8. pp. 2451-2463.

Bibtex

@article{67b25d30db3a4a4e9a0d1ec559fe1623,
title = "New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen",
abstract = "Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS-PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS-green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI.",
keywords = "Actin, actin-binding proteins (ABPs), calcium, fluorescent probes, programmed cell death (PCD), pH, pollen tube growth, live-cell imaging, self-incompatibility (SI), endocytosis",
author = "Ludi Wang and Marina Trivi{\~n}o and Zongcheng Lin and Jos{\'e} Carli and Eaves, {Deborah J} and {Van Damme}, Daniёl and Nowack, {Moritz K} and Franklin-Tong, {Vernonica E} and Maurice Bosch",
note = "{\textcopyright} The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.",
year = "2020",
month = apr,
day = "23",
doi = "10.1093/jxb/eraa092",
language = "English",
volume = "71",
pages = "2451--2463",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen

AU - Wang, Ludi

AU - Triviño, Marina

AU - Lin, Zongcheng

AU - Carli, José

AU - Eaves, Deborah J

AU - Van Damme, Daniёl

AU - Nowack, Moritz K

AU - Franklin-Tong, Vernonica E

AU - Bosch, Maurice

N1 - © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

PY - 2020/4/23

Y1 - 2020/4/23

N2 - Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS-PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS-green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI.

AB - Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilization. Mechanistically, one of the best-studied SI systems is that of Papaver rhoeas (poppy). This utilizes two S-determinants: stigma-expressed PrsS and pollen-expressed PrpS. Interaction of cognate PrpS-PrsS triggers a signalling network, causing rapid growth arrest and programmed cell death (PCD) in incompatible pollen. We previously demonstrated that transgenic Arabidopsis thaliana pollen expressing PrpS-green fluorescent protein (GFP) can respond to Papaver PrsS with remarkably similar responses to those observed in incompatible Papaver pollen. Here we describe recent advances using these transgenic plants combined with genetically encoded fluorescent probes to monitor SI-induced cellular alterations, including cytosolic calcium, pH, the actin cytoskeleton, clathrin-mediated endocytosis (CME), and the vacuole. This approach has allowed us to study the SI response in depth, using multiparameter live-cell imaging approaches that were not possible in Papaver. This lays the foundations for new opportunities to elucidate key mechanisms involved in SI. Here we establish that CME is disrupted in self-incompatible pollen. Moreover, we reveal new detailed information about F-actin remodelling in pollen tubes after SI.

KW - Actin

KW - actin-binding proteins (ABPs)

KW - calcium

KW - fluorescent probes

KW - programmed cell death (PCD)

KW - pH

KW - pollen tube growth

KW - live-cell imaging

KW - self-incompatibility (SI)

KW - endocytosis

U2 - 10.1093/jxb/eraa092

DO - 10.1093/jxb/eraa092

M3 - Article

C2 - 32100005

VL - 71

SP - 2451

EP - 2463

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 8

ER -