Neutrophil superoxide release and plasma C-reactive protein levels pre- and post-periodontal therapy

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@article{892677a6d420435bbc3fc17a521588e9,
title = "Neutrophil superoxide release and plasma C-reactive protein levels pre- and post-periodontal therapy",
abstract = "AimTo determine peripheral blood neutrophil superoxide release and C-reactive protein (CRP) concentration in chronic periodontitis patients, before and after non-surgical periodontal treatment.Materials and MethodsNeutrophils were isolated from patient and control volunteers (n = 20) and superoxide measured by lucigenin-enhanced chemiluminescence with and without stimulation with unopsonized Porphyromonas gingivalis, unopsonized Fusobacterium nucleatum and phorbol 12-myristate 13-acetate (PMA) before and 2-months following non-surgical therapy. Corresponding high-sensitivity plasma CRP concentrations were also determined.ResultsAt pre-treatment baseline, patient neutrophils released more superoxide in the absence (p ≤ 0.032) and presence of periodontal bacteria (p ≤ 0.013) and after PMA stimulation (p = 0.041) compared to control cells. Post-therapy, patient neutrophil superoxide release was reduced to control cell levels. Median patient plasma CRP concentrations were non-significantly higher than control values and were reduced after therapy (1.80–1.36 mg/l). Patient pre-treatment baseline, unstimulated neutrophil superoxide release showed a significant, positive correlation with plasma CRP concentration (p = 0.01).ConclusionsChronic periodontitis is characterized by peripheral neutrophils exhibiting superoxide hyperactivity and hyper-reactivity to periodontal pathogens that is not a constitutive feature of periodontitis patients. The positive, pre-therapy relationship between unstimulated neutrophil superoxide release and plasma CRP is consistent with a protective role for CRP in reducing oxidative stress and systemic inflammation in vivo.",
author = "Martin Ling and Iain Chapple and John Matthews",
year = "2016",
month = aug,
doi = "10.1111/jcpe.12575",
language = "English",
volume = "43",
pages = "652--658",
journal = "Journal of Clinical Periodontology",
issn = "0303-6979",
publisher = "Wiley",
number = "8",

}

RIS

TY - JOUR

T1 - Neutrophil superoxide release and plasma C-reactive protein levels pre- and post-periodontal therapy

AU - Ling, Martin

AU - Chapple, Iain

AU - Matthews, John

PY - 2016/8

Y1 - 2016/8

N2 - AimTo determine peripheral blood neutrophil superoxide release and C-reactive protein (CRP) concentration in chronic periodontitis patients, before and after non-surgical periodontal treatment.Materials and MethodsNeutrophils were isolated from patient and control volunteers (n = 20) and superoxide measured by lucigenin-enhanced chemiluminescence with and without stimulation with unopsonized Porphyromonas gingivalis, unopsonized Fusobacterium nucleatum and phorbol 12-myristate 13-acetate (PMA) before and 2-months following non-surgical therapy. Corresponding high-sensitivity plasma CRP concentrations were also determined.ResultsAt pre-treatment baseline, patient neutrophils released more superoxide in the absence (p ≤ 0.032) and presence of periodontal bacteria (p ≤ 0.013) and after PMA stimulation (p = 0.041) compared to control cells. Post-therapy, patient neutrophil superoxide release was reduced to control cell levels. Median patient plasma CRP concentrations were non-significantly higher than control values and were reduced after therapy (1.80–1.36 mg/l). Patient pre-treatment baseline, unstimulated neutrophil superoxide release showed a significant, positive correlation with plasma CRP concentration (p = 0.01).ConclusionsChronic periodontitis is characterized by peripheral neutrophils exhibiting superoxide hyperactivity and hyper-reactivity to periodontal pathogens that is not a constitutive feature of periodontitis patients. The positive, pre-therapy relationship between unstimulated neutrophil superoxide release and plasma CRP is consistent with a protective role for CRP in reducing oxidative stress and systemic inflammation in vivo.

AB - AimTo determine peripheral blood neutrophil superoxide release and C-reactive protein (CRP) concentration in chronic periodontitis patients, before and after non-surgical periodontal treatment.Materials and MethodsNeutrophils were isolated from patient and control volunteers (n = 20) and superoxide measured by lucigenin-enhanced chemiluminescence with and without stimulation with unopsonized Porphyromonas gingivalis, unopsonized Fusobacterium nucleatum and phorbol 12-myristate 13-acetate (PMA) before and 2-months following non-surgical therapy. Corresponding high-sensitivity plasma CRP concentrations were also determined.ResultsAt pre-treatment baseline, patient neutrophils released more superoxide in the absence (p ≤ 0.032) and presence of periodontal bacteria (p ≤ 0.013) and after PMA stimulation (p = 0.041) compared to control cells. Post-therapy, patient neutrophil superoxide release was reduced to control cell levels. Median patient plasma CRP concentrations were non-significantly higher than control values and were reduced after therapy (1.80–1.36 mg/l). Patient pre-treatment baseline, unstimulated neutrophil superoxide release showed a significant, positive correlation with plasma CRP concentration (p = 0.01).ConclusionsChronic periodontitis is characterized by peripheral neutrophils exhibiting superoxide hyperactivity and hyper-reactivity to periodontal pathogens that is not a constitutive feature of periodontitis patients. The positive, pre-therapy relationship between unstimulated neutrophil superoxide release and plasma CRP is consistent with a protective role for CRP in reducing oxidative stress and systemic inflammation in vivo.

U2 - 10.1111/jcpe.12575

DO - 10.1111/jcpe.12575

M3 - Article

VL - 43

SP - 652

EP - 658

JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

IS - 8

ER -