Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action

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Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action. / Esteban-Fernández, A; Rendeiro, Catarina; Spencer, J P E; Del Coso, D Gigorro; de Llano, M D González; Bartolomé, B; Moreno-Arribas, M V.

In: Frontiers in Nutrition, Vol. 4, 14.03.2017, p. 3.

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Esteban-Fernández, A ; Rendeiro, Catarina ; Spencer, J P E ; Del Coso, D Gigorro ; de Llano, M D González ; Bartolomé, B ; Moreno-Arribas, M V. / Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action. In: Frontiers in Nutrition. 2017 ; Vol. 4. pp. 3.

Bibtex

@article{fb48f89919904034b09cd56ed9bc2c94,
title = "Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action",
abstract = "Moderate wine consumption has shown the potential to delay the onset of neurodegenerative diseases. This study investigates the molecular mechanisms underlying the protective effects of wine-derived phenolic and aroma compounds in a neuroinflammation model based on SIN-1 stress-induced injury in SH-SY5Y neuroblastoma cells. Cell pretreatment with microbial metabolites found in blood after wine consumption, 3,4-dihydroxyphenylacetic (3,4-DHPA), 3-hydroxyphenylacetic acids and salicylic β-d-O-glucuronide, at physiologically concentrations (0.1-10 μM) resulted in increased cell viability versus SIN-1 control group (p < 0.05). Results also showed significant decreases in mitogen-activated protein kinase (MAPK) p38 and ERK1/2 activation as well as in downstream pro-apoptotic caspase-3 activity by some of the studied compounds. Moreover, pretreatment with p38, MEK, and ERK1/2-specific inhibitors, which have a phenolic-like structure, also resulted in an increase on cell survival and a reduction on caspase-3 activity levels. Overall, these results contribute with new evidences related to the neuroprotective actions of wine, pointing out that wine-derived human metabolites and aroma compounds may be effective at protecting neuroblastoma cells from nitrosative stress injury by inhibiting neuronal MAPK p38 and ERK1/2, as well as downstream caspase 3 activity.",
keywords = "mitogen-activated protein kinase, neuroprotection, wine, polyphenols, gut phenolic metabolites, aroma compounds",
author = "A Esteban-Fern{\'a}ndez and Catarina Rendeiro and Spencer, {J P E} and {Del Coso}, {D Gigorro} and {de Llano}, {M D Gonz{\'a}lez} and B Bartolom{\'e} and Moreno-Arribas, {M V}",
year = "2017",
month = mar,
day = "14",
doi = "10.3389/fnut.2017.00003",
language = "English",
volume = "4",
pages = "3",
journal = "Frontiers in Nutrition",
issn = "2296-861X",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action

AU - Esteban-Fernández, A

AU - Rendeiro, Catarina

AU - Spencer, J P E

AU - Del Coso, D Gigorro

AU - de Llano, M D González

AU - Bartolomé, B

AU - Moreno-Arribas, M V

PY - 2017/3/14

Y1 - 2017/3/14

N2 - Moderate wine consumption has shown the potential to delay the onset of neurodegenerative diseases. This study investigates the molecular mechanisms underlying the protective effects of wine-derived phenolic and aroma compounds in a neuroinflammation model based on SIN-1 stress-induced injury in SH-SY5Y neuroblastoma cells. Cell pretreatment with microbial metabolites found in blood after wine consumption, 3,4-dihydroxyphenylacetic (3,4-DHPA), 3-hydroxyphenylacetic acids and salicylic β-d-O-glucuronide, at physiologically concentrations (0.1-10 μM) resulted in increased cell viability versus SIN-1 control group (p < 0.05). Results also showed significant decreases in mitogen-activated protein kinase (MAPK) p38 and ERK1/2 activation as well as in downstream pro-apoptotic caspase-3 activity by some of the studied compounds. Moreover, pretreatment with p38, MEK, and ERK1/2-specific inhibitors, which have a phenolic-like structure, also resulted in an increase on cell survival and a reduction on caspase-3 activity levels. Overall, these results contribute with new evidences related to the neuroprotective actions of wine, pointing out that wine-derived human metabolites and aroma compounds may be effective at protecting neuroblastoma cells from nitrosative stress injury by inhibiting neuronal MAPK p38 and ERK1/2, as well as downstream caspase 3 activity.

AB - Moderate wine consumption has shown the potential to delay the onset of neurodegenerative diseases. This study investigates the molecular mechanisms underlying the protective effects of wine-derived phenolic and aroma compounds in a neuroinflammation model based on SIN-1 stress-induced injury in SH-SY5Y neuroblastoma cells. Cell pretreatment with microbial metabolites found in blood after wine consumption, 3,4-dihydroxyphenylacetic (3,4-DHPA), 3-hydroxyphenylacetic acids and salicylic β-d-O-glucuronide, at physiologically concentrations (0.1-10 μM) resulted in increased cell viability versus SIN-1 control group (p < 0.05). Results also showed significant decreases in mitogen-activated protein kinase (MAPK) p38 and ERK1/2 activation as well as in downstream pro-apoptotic caspase-3 activity by some of the studied compounds. Moreover, pretreatment with p38, MEK, and ERK1/2-specific inhibitors, which have a phenolic-like structure, also resulted in an increase on cell survival and a reduction on caspase-3 activity levels. Overall, these results contribute with new evidences related to the neuroprotective actions of wine, pointing out that wine-derived human metabolites and aroma compounds may be effective at protecting neuroblastoma cells from nitrosative stress injury by inhibiting neuronal MAPK p38 and ERK1/2, as well as downstream caspase 3 activity.

KW - mitogen-activated protein kinase

KW - neuroprotection

KW - wine

KW - polyphenols

KW - gut phenolic metabolites

KW - aroma compounds

U2 - 10.3389/fnut.2017.00003

DO - 10.3389/fnut.2017.00003

M3 - Article

C2 - 28352628

VL - 4

SP - 3

JO - Frontiers in Nutrition

JF - Frontiers in Nutrition

SN - 2296-861X

ER -