TY - JOUR
T1 - Neonatal and adult recent thymic emigrants produce IL-8 and express complement receptors CR1 and CR2
AU - Pekalski, Marcin L
AU - García, Arcadio Rubio
AU - Ferreira, Ricardo C
AU - Rainbow, Daniel B
AU - Smyth, Deborah J
AU - Mashar, Meghavi
AU - Brady, Jane
AU - Savinykh, Natalia
AU - Dopico, Xaquin Castro
AU - Mahmood, Sumiyya
AU - Duley, Simon
AU - Stevens, Helen E
AU - Walker, Neil M
AU - Cutler, Antony J
AU - Waldron-Lynch, Frank
AU - Dunger, David B
AU - Shannon-Lowe, Claire
AU - Coles, Alasdair J
AU - Jones, Joanne L
AU - Wallace, Chris
AU - Todd, John A
AU - Wicker, Linda S
PY - 2017/8/17
Y1 - 2017/8/17
N2 - The maintenance of peripheral naive T lymphocytes in humans is dependent on their homeostatic division, not continuing emigration from the thymus, which undergoes involution with age. However, postthymic maintenance of naive T cells is still poorly understood. Previously we reported that recent thymic emigrants (RTEs) are contained in CD31+CD25- naive T cells as defined by their levels of signal joint T cell receptor rearrangement excision circles (sjTRECs). Here, by differential gene expression analysis followed by protein expression and functional studies, we define that the naive T cells having divided the least since thymic emigration express complement receptors (CR1 and CR2) known to bind complement C3b- and C3d-decorated microbial products and, following activation, produce IL-8 (CXCL8), a major chemoattractant for neutrophils in bacterial defense. We also observed an IL-8-producing memory T cell subpopulation coexpressing CR1 and CR2 and with a gene expression signature resembling that of RTEs. The functions of CR1 and CR2 on T cells remain to be determined, but we note that CR2 is the receptor for Epstein-Barr virus, which is a cause of T cell lymphomas and a candidate environmental factor in autoimmune disease.
AB - The maintenance of peripheral naive T lymphocytes in humans is dependent on their homeostatic division, not continuing emigration from the thymus, which undergoes involution with age. However, postthymic maintenance of naive T cells is still poorly understood. Previously we reported that recent thymic emigrants (RTEs) are contained in CD31+CD25- naive T cells as defined by their levels of signal joint T cell receptor rearrangement excision circles (sjTRECs). Here, by differential gene expression analysis followed by protein expression and functional studies, we define that the naive T cells having divided the least since thymic emigration express complement receptors (CR1 and CR2) known to bind complement C3b- and C3d-decorated microbial products and, following activation, produce IL-8 (CXCL8), a major chemoattractant for neutrophils in bacterial defense. We also observed an IL-8-producing memory T cell subpopulation coexpressing CR1 and CR2 and with a gene expression signature resembling that of RTEs. The functions of CR1 and CR2 on T cells remain to be determined, but we note that CR2 is the receptor for Epstein-Barr virus, which is a cause of T cell lymphomas and a candidate environmental factor in autoimmune disease.
U2 - 10.1172/jci.insight.93739
DO - 10.1172/jci.insight.93739
M3 - Article
C2 - 28814669
SN - 2379-3708
VL - 2
JO - JCI Insight
JF - JCI Insight
IS - 16
ER -