Neonatal and adult CD4+CD3- cells share similar gene expression profile, and neonatal cells upregulate OX40-ligand in response to TL1A (TNFSF15)
Research output: Contribution to journal › Article
Authors
Colleges, School and Institutes
Abstract
We report here the quantitative expression of a set of immunity-related genes, including TNF family members, chemokine receptors, and transcription factors, in a CD4(+)CD3(-) accessory cell. By correlating gene expression between cell-sorted populations of defined phenotype, we show that the genetic fingerprint of these CD4(+)CD3(-) cells is distinct from dendritic cells, plasmacytoid dendritic cells, T cells, B cells, and NK cells. In contrast, it is highly similar to CD4(+)CD3(-) cells isolated from embryonic and neonatal tissues, with the exception that only adult populations express OX40L and CD30L. We have previously reported that IL-7 signals regulate CD30L expression. In the present study, we show that both neonatal and adult CD4(+)CD3(-) cells express the TNF family member, death receptor 3 (TNFRSF25), and that addition of TL1A (TNFSF15), the ligand for death receptor 3, up-regulates OX40L on neonatal CD4(+)CD3(-) cells. Finally, we demonstrate that this differentiation occurs in vivo: neonatal CD4(+)CD3(-) cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient.
Details
Original language | English |
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Pages (from-to) | 3074-3081 |
Number of pages | 8 |
Journal | Journal of Immunology |
Volume | 177 |
Publication status | Published - 1 Jan 2006 |