MYOD-1 in normal colonic mucosa--role as a putative biomarker?

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MYOD-1 in normal colonic mucosa--role as a putative biomarker? / Arasaradnam, Ramesh P; Quraishi, M Nabil; Commane, Daniel; Mathers, John C; Bradburn, Mike.

In: BMC Research Notes, Vol. 5, 11.07.2012, p. 240.

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Arasaradnam, Ramesh P ; Quraishi, M Nabil ; Commane, Daniel ; Mathers, John C ; Bradburn, Mike. / MYOD-1 in normal colonic mucosa--role as a putative biomarker?. In: BMC Research Notes. 2012 ; Vol. 5. pp. 240.

Bibtex

@article{7e4e31bab5684ca9a4634efc97bb818b,
title = "MYOD-1 in normal colonic mucosa--role as a putative biomarker?",
abstract = "BACKGROUND: DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.RESULTS: We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p<0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio - the latter is also a known risk factor for colon cancer development.CONCLUSIONS: Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.",
keywords = "Adult, Aging/genetics, Anthropometry, Biomarkers/metabolism, Colon/metabolism, DNA Methylation/genetics, Female, Humans, Intestinal Mucosa/metabolism, Life Style, Male, Middle Aged, MyoD Protein/genetics",
author = "Arasaradnam, {Ramesh P} and Quraishi, {M Nabil} and Daniel Commane and Mathers, {John C} and Mike Bradburn",
year = "2012",
month = jul,
day = "11",
doi = "10.1186/1756-0500-5-240",
language = "English",
volume = "5",
pages = "240",
journal = "BMC Research Notes",
issn = "1756-0500",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - MYOD-1 in normal colonic mucosa--role as a putative biomarker?

AU - Arasaradnam, Ramesh P

AU - Quraishi, M Nabil

AU - Commane, Daniel

AU - Mathers, John C

AU - Bradburn, Mike

PY - 2012/7/11

Y1 - 2012/7/11

N2 - BACKGROUND: DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.RESULTS: We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p<0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio - the latter is also a known risk factor for colon cancer development.CONCLUSIONS: Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.

AB - BACKGROUND: DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is an age related event. However the role of this gene in the colonic mucosa is not understood and whether methylation occurs in subjects without colon cancer. In this study, we have determined the frequency of methylation of the MYOD-1 gene in normal colonic mucosa and investigated to see if this is associated with established colorectal cancer risk factors primarily ageing.RESULTS: We analysed colonic mucosal biopsies in 218 normal individuals and demonstrated that in most individuals promoter hypermethylation was not quantified for MYOD-1. However, promoter hypermethylation increased significantly with age (p<0.001 using regression analysis) and this was gender independent. We also showed that gene promoter methylation increased positively with an increase in waist to hip (WHR) ratio - the latter is also a known risk factor for colon cancer development.CONCLUSIONS: Our study suggests that promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer.

KW - Adult

KW - Aging/genetics

KW - Anthropometry

KW - Biomarkers/metabolism

KW - Colon/metabolism

KW - DNA Methylation/genetics

KW - Female

KW - Humans

KW - Intestinal Mucosa/metabolism

KW - Life Style

KW - Male

KW - Middle Aged

KW - MyoD Protein/genetics

U2 - 10.1186/1756-0500-5-240

DO - 10.1186/1756-0500-5-240

M3 - Article

C2 - 22591756

VL - 5

SP - 240

JO - BMC Research Notes

JF - BMC Research Notes

SN - 1756-0500

ER -