Muscle protein breakdown is impaired during immobilization compared to during a subsequent retraining period in older men: no effect of anti-inflammatory medication

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Muscle protein breakdown is impaired during immobilization compared to during a subsequent retraining period in older men : no effect of anti-inflammatory medication. / Dideriksen, K; Reitelseder, S; Agergaard, J; Boesen, A P; Aas, S N; Raastad, T; Holm, Lars.

In: Pfluegers Archiv: European journal of physiology , Vol. 472, No. 2, 05.02.2020, p. 281-292.

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@article{82cea891f65642f693ac051b4cd3567a,
title = "Muscle protein breakdown is impaired during immobilization compared to during a subsequent retraining period in older men: no effect of anti-inflammatory medication",
abstract = "Muscle inactivity reduces muscle protein synthesis (MPS), whereas a subsequent period of rehabilitation resistance training (retraining) increases MPS. However, less is known regarding muscle protein breakdown (MPB) during such conditions. Furthermore, nonsteroidal anti-inflammatory drugs (NSAIDs) may have a dampening effect on MPB during periods of inactivity in older individuals. Thus, we measured the average MPB, by use of the deuterated water methodology, during an immobilization period and a subsequent retraining period in older individuals with and without NSAID treatment. Eighteen men (60-80 years: range) were randomly assigned to ibuprofen (1200 mg/d, Ibu) or placebo (Plc). One lower limb was immobilized in a cast for 2 weeks and retrained for 2 weeks, and 2 × 20 g of whey protein was ingested daily during both periods. Besides MPB, the protein expression of different muscle degradation signaling molecules was investigated. MPB was lower during immobilization compared to retraining (p < 0.01). NSAID treatment did not affect the MPB rate during immobilization or retraining (p > 0.05). The protein expression of muscle degradation signaling molecules changed during the study intervention but were unaffected by NSAID treatment. The finding that MPB was lower during immobilization than during retraining indicates that an increased MPB may play an important role in the muscle protein remodeling processes taking place within the initial retraining period. Moreover, NSAID treatment did not significantly influence the MPB rate during 2 weeks of lower limb immobilization or during 2 weeks of subsequent retraining in older individuals.",
keywords = "Deuterated alanine, Deuterated water, Muscle degradation, Muscle disuse, Muscle recovery, NSAID",
author = "K Dideriksen and S Reitelseder and J Agergaard and Boesen, {A P} and Aas, {S N} and T Raastad and Lars Holm",
year = "2020",
month = feb,
day = "5",
doi = "10.1007/s00424-020-02353-w",
language = "English",
volume = "472",
pages = "281--292",
journal = "Pfluegers Archiv: European journal of physiology ",
issn = "0031-6768",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Muscle protein breakdown is impaired during immobilization compared to during a subsequent retraining period in older men

T2 - no effect of anti-inflammatory medication

AU - Dideriksen, K

AU - Reitelseder, S

AU - Agergaard, J

AU - Boesen, A P

AU - Aas, S N

AU - Raastad, T

AU - Holm, Lars

PY - 2020/2/5

Y1 - 2020/2/5

N2 - Muscle inactivity reduces muscle protein synthesis (MPS), whereas a subsequent period of rehabilitation resistance training (retraining) increases MPS. However, less is known regarding muscle protein breakdown (MPB) during such conditions. Furthermore, nonsteroidal anti-inflammatory drugs (NSAIDs) may have a dampening effect on MPB during periods of inactivity in older individuals. Thus, we measured the average MPB, by use of the deuterated water methodology, during an immobilization period and a subsequent retraining period in older individuals with and without NSAID treatment. Eighteen men (60-80 years: range) were randomly assigned to ibuprofen (1200 mg/d, Ibu) or placebo (Plc). One lower limb was immobilized in a cast for 2 weeks and retrained for 2 weeks, and 2 × 20 g of whey protein was ingested daily during both periods. Besides MPB, the protein expression of different muscle degradation signaling molecules was investigated. MPB was lower during immobilization compared to retraining (p < 0.01). NSAID treatment did not affect the MPB rate during immobilization or retraining (p > 0.05). The protein expression of muscle degradation signaling molecules changed during the study intervention but were unaffected by NSAID treatment. The finding that MPB was lower during immobilization than during retraining indicates that an increased MPB may play an important role in the muscle protein remodeling processes taking place within the initial retraining period. Moreover, NSAID treatment did not significantly influence the MPB rate during 2 weeks of lower limb immobilization or during 2 weeks of subsequent retraining in older individuals.

AB - Muscle inactivity reduces muscle protein synthesis (MPS), whereas a subsequent period of rehabilitation resistance training (retraining) increases MPS. However, less is known regarding muscle protein breakdown (MPB) during such conditions. Furthermore, nonsteroidal anti-inflammatory drugs (NSAIDs) may have a dampening effect on MPB during periods of inactivity in older individuals. Thus, we measured the average MPB, by use of the deuterated water methodology, during an immobilization period and a subsequent retraining period in older individuals with and without NSAID treatment. Eighteen men (60-80 years: range) were randomly assigned to ibuprofen (1200 mg/d, Ibu) or placebo (Plc). One lower limb was immobilized in a cast for 2 weeks and retrained for 2 weeks, and 2 × 20 g of whey protein was ingested daily during both periods. Besides MPB, the protein expression of different muscle degradation signaling molecules was investigated. MPB was lower during immobilization compared to retraining (p < 0.01). NSAID treatment did not affect the MPB rate during immobilization or retraining (p > 0.05). The protein expression of muscle degradation signaling molecules changed during the study intervention but were unaffected by NSAID treatment. The finding that MPB was lower during immobilization than during retraining indicates that an increased MPB may play an important role in the muscle protein remodeling processes taking place within the initial retraining period. Moreover, NSAID treatment did not significantly influence the MPB rate during 2 weeks of lower limb immobilization or during 2 weeks of subsequent retraining in older individuals.

KW - Deuterated alanine

KW - Deuterated water

KW - Muscle degradation

KW - Muscle disuse

KW - Muscle recovery

KW - NSAID

UR - http://www.scopus.com/inward/record.url?scp=85079139494&partnerID=8YFLogxK

U2 - 10.1007/s00424-020-02353-w

DO - 10.1007/s00424-020-02353-w

M3 - Article

C2 - 32025814

VL - 472

SP - 281

EP - 292

JO - Pfluegers Archiv: European journal of physiology

JF - Pfluegers Archiv: European journal of physiology

SN - 0031-6768

IS - 2

ER -