Projects per year
Abstract
Two major monocyte subsets, CD14+CD16- (classical) and CD14+/dimCD16+ (nonclassical/intermediate), have been described. Each has different functions ascribed in its interactions with vascular endothelial cells (EC), including migration and promoting inflammation. Although monocyte subpopulations have been studied in isolated systems, their influence on EC and on the course of inflammation has been ignored. In this study, using unstimulated or cytokine-activated EC, we observed significant differences in the recruitment, migration, and reverse migration of human monocyte subsets. Associated with this, and based on their patterns of cytokine secretion, there was a difference in their capacity to activate EC and support the secondary recruitment of flowing neutrophils. High levels of TNF were detected in cocultures with nonclassical/intermediate monocytes, the blockade of which significantly reduced neutrophil recruitment. In contrast, classical monocytes secreted high levels of IL-6, the blockade of which resulted in increased neutrophil recruitment. When cocultures contained both monocyte subsets, or when conditioned supernatant from classical monocytes cocultures (IL-6hi) was added to nonclassical/intermediate monocyte cocultures (TNFhi), the activating effects of TNF were dramatically reduced, implying that when present, the anti-inflammatory activities of IL-6 were dominant over the proinflammatory activities of TNF. These changes in neutrophil recruitment could be explained by regulation of E-selectin on the cocultured EC. This study suggests that recruited human monocyte subsets trigger a regulatory pathway of cytokine-mediated signaling at the EC interface, and we propose that this is a mechanism for limiting the phlogistic activity of newly recruited monocytes.
Original language | English |
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Pages (from-to) | 2834-2843 |
Journal | Journal of Immunology |
Volume | 198 |
Issue number | 7 |
Early online date | 13 Feb 2017 |
DOIs | |
Publication status | Published - 1 Apr 2017 |
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- 5 Finished
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BHF Studentship - The role of platelet derived microvesicles in regulating the differentiation and function of foam cells
Rainger, E. & Nash, G.
5/01/15 → 4/01/18
Project: Research
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Do Platelets Exacerbate the Atherogenic Process by Regulating the Recruitment, Differentiation and Inflammatory Function of Monocytes
Rainger, E., Nash, G. & Watson, S.
1/09/12 → 31/08/17
Project: Research
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The Role of Src-Family Kinases in Regulating Transforming Growth Factor-B1 Driven Platelet and Monocyte Recruitment to Endothelial Cells
Rainger, E., Watson, S. & Nash, G.
1/10/11 → 30/09/14
Project: Research
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The Ability of Fatty Acids to Modulate the Inflammatory Effects of Macrophage/Monocytes on Endothelial Cells
Rainger, E. & Nash, G.
Biotechnology & Biological Sciences Research Council
1/04/04 → 31/05/07
Project: Research Councils