Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51

Research output: Contribution to journalArticle

Authors

  • B Alsayyed
  • S Piperdi
  • X Yuan
  • [No Value] [No Value]
  • W Jacobsjr
  • A Casadevall
  • A Glatmanfreedman

Colleges, School and Institutes

Abstract

Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular potysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobutin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell watt, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell. (c) 2007 Elsevier Ltd. All rights reserved.

Details

Original languageEnglish
Pages (from-to)489-497
Number of pages9
JournalTuberculosis
Volume87
Issue number6
Publication statusPublished - 1 Nov 2007

Keywords

  • monoclonal, antibodies, MPT51, Mycobacterium tuberculosis