Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis

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Colleges, School and Institutes

Abstract

Ser/Thr phosphorylation has emerged as a critical regulatory mechanism in a number of bacteria, including Mycobacterium tuberculosis. This problematic pathogen encodes 11 eukaryotic-like Ser/Thr kinases, yet few substrates or signaling targets have been characterized. Here, we report the structure of EmbR (2.0 angstrom), a putative transcriptional regulator of key arabinosyltransferases (EmbC, -A, and -B), and an endogenous substrate of the Ser/Thrkinase PknH. EmbR presents a unique domain architecture: the N-terminal winged-helix DNA-binding domain forms an extensive interface with the all-helical central bacterial transcriptional activation domain and is positioned adjacent to the regulatory C-terminal forkhead-associated (FHA) domain, which mediates binding to a Thr-phosphorylated site in PknH. The structure in complex with a phospho-peptide (1.9 angstrom) reveals a conserved mode of phospho-threonine recognition by the FHA domain and evidence for specific recognition of the cognate kinase. The present structures suggest hypotheses as to how EmbR might propagate the phospho-relay signal from its cognate kinase, while serving as a template for the structurally uncharacterized Streptomyces antibiotic regulatory protein family of transcription factors.

Details

Original languageEnglish
Pages (from-to)2558-2563
Number of pages6
JournalNational Academy of Sciences. Proceedings
Volume103
Issue number8
Publication statusPublished - 6 Feb 2006

Keywords

  • transcriptional regulator, ethambutol, arabinosyltransferase, x-ray crystallography, PknH