Molecular recognition determinants for type IV secretion of diverse families of conjugative relaxases

Research output: Contribution to journalArticle

Authors

  • Silvia Lang
  • Karl Gruber
  • Sanja Mihajlovic
  • Christian J Gruber
  • Sonja Steinlechner
  • Marc-Andre Jehl
  • Thomas Rattei
  • Kai-Uwe Fröhlich
  • Ellen L Zechner

Colleges, School and Institutes

External organisations

  • University of Graz, Institute of Molecular Biosciences, Humboldtstrasse 50, A-8010 Graz, Austria.

Abstract

In preparation for transfer conjugative type IV secretion systems (T4SS) produce a nucleoprotein adduct containing a relaxase enzyme covalently linked to the 5' end of single-stranded plasmid DNA. The bound relaxase is expected to present features necessary for selective recognition by the type IV coupling protein (T4CP), which controls substrate entry to the envelope spanning secretion machinery. We prove that the IncF plasmid R1 relaxase TraI is translocated to the recipient cells. Using a Cre recombinase assay (CRAfT) we mapped two internally positioned translocation signals (TS) on F-like TraI proteins that independently mediate efficient recognition and secretion. Tertiary structure predictions for the TS matched best helicase RecD2 from Deinococcus radiodurans. The TS is widely conserved in MOB(F) and MOB(Q) families of relaxases. Structure/function relationships within the TS were identified by mutation. A key residue in specific recognition by T4CP TraD was revealed by a fidelity switch phenotype for an F to plasmid R1 exchange L626H mutation. Finally, we show that physical linkage of the relaxase catalytic domain to a TraI TS is necessary for efficient conjugative transfer.

Details

Original languageEnglish
Pages (from-to)1539-55
Number of pages17
JournalMolecular Microbiology
Volume78
Issue number6
Publication statusPublished - Dec 2010

Keywords

  • Conjugation, Genetic, DNA Helicases, Escherichia coli, Escherichia coli Proteins, Multigene Family, Protein Sorting Signals, Protein Structure, Tertiary, Protein Transport, Journal Article, Research Support, Non-U.S. Gov't