Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data

Research output: Contribution to journalArticle

Authors

  • Andrew Cope
  • Dylan Owen
  • George Ashdown
  • Paul W. Wiseman

Colleges, School and Institutes

External organisations

  • Division of Immunology, Infection and Inflammatory Disease, Academic Department of Rheumatology, King's College, London
  • Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College, London
  • Departments of Chemistry and Physics, McGill University

Abstract

We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.

Details

Original languageEnglish
Pages (from-to)L21-L23
JournalBiophysical Journal
Volume107
Issue number9
Publication statusPublished - 16 Sep 2014

Keywords

  • ACTIN POLYMERIZATION, RETROGRADE FLOW, T-CELLS