Molecular flow quantified beyond the diffraction limit by spatiotemporal image correlation of structured illumination microscopy data

Research output: Contribution to journalLetterpeer-review


  • Andrew Cope
  • Dylan Owen
  • George Ashdown
  • Paul W. Wiseman

Colleges, School and Institutes

External organisations

  • Division of Immunology, Infection and Inflammatory Disease, Academic Department of Rheumatology, King's College, London
  • Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College, London
  • McGill University


We combine total internal reflection fluorescence structured illumination microscopy with spatiotemporal image correlation spectroscopy to quantify the flow velocities and directionality of filamentous-actin at the T cell immunological synapse. These techniques demonstrate it is possible to image retrograde flow of filamentous-actin at superresolution and provide flow quantification in the form of velocity histograms and flow vector maps. The flow was found to be retrograde and radially directed throughout the periphery of T-cells during synapse formation.


Original languageEnglish
Pages (from-to)L21-L23
Number of pages3
JournalBiophysical Journal
Issue number9
Publication statusPublished - 4 Nov 2014


  • actin polymerization, retrograde flow, T-cells