Modulation of Shigella virulence in response to available oxygen in vivo

Research output: Contribution to journalArticle

Standard

Modulation of Shigella virulence in response to available oxygen in vivo. / Marteyn, B; West, NP; Browning, Douglas; Cole, Jeffrey; Shaw, JG; Palm, F; Mounier, J; Prevost, MC; Sansonetti, P; Tang, CM.

In: Nature, Vol. 465, No. 7296, 01.05.2010, p. 355-U113.

Research output: Contribution to journalArticle

Harvard

Marteyn, B, West, NP, Browning, D, Cole, J, Shaw, JG, Palm, F, Mounier, J, Prevost, MC, Sansonetti, P & Tang, CM 2010, 'Modulation of Shigella virulence in response to available oxygen in vivo', Nature, vol. 465, no. 7296, pp. 355-U113. https://doi.org/10.1038/nature08970

APA

Marteyn, B., West, NP., Browning, D., Cole, J., Shaw, JG., Palm, F., Mounier, J., Prevost, MC., Sansonetti, P., & Tang, CM. (2010). Modulation of Shigella virulence in response to available oxygen in vivo. Nature, 465(7296), 355-U113. https://doi.org/10.1038/nature08970

Vancouver

Author

Marteyn, B ; West, NP ; Browning, Douglas ; Cole, Jeffrey ; Shaw, JG ; Palm, F ; Mounier, J ; Prevost, MC ; Sansonetti, P ; Tang, CM. / Modulation of Shigella virulence in response to available oxygen in vivo. In: Nature. 2010 ; Vol. 465, No. 7296. pp. 355-U113.

Bibtex

@article{bd6bd44e299449cd8df0c2398cc183ba,
title = "Modulation of Shigella virulence in response to available oxygen in vivo",
abstract = "Bacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence(1). In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence.",
author = "B Marteyn and NP West and Douglas Browning and Jeffrey Cole and JG Shaw and F Palm and J Mounier and MC Prevost and P Sansonetti and CM Tang",
year = "2010",
month = may,
day = "1",
doi = "10.1038/nature08970",
language = "English",
volume = "465",
pages = "355--U113",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7296",

}

RIS

TY - JOUR

T1 - Modulation of Shigella virulence in response to available oxygen in vivo

AU - Marteyn, B

AU - West, NP

AU - Browning, Douglas

AU - Cole, Jeffrey

AU - Shaw, JG

AU - Palm, F

AU - Mounier, J

AU - Prevost, MC

AU - Sansonetti, P

AU - Tang, CM

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Bacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence(1). In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence.

AB - Bacteria coordinate expression of virulence determinants in response to localized microenvironments in their hosts. Here we show that Shigella flexneri, which causes dysentery, encounters varying oxygen concentrations in the gastrointestinal tract, which govern activity of its type three secretion system (T3SS). The T3SS is essential for cell invasion and virulence(1). In anaerobic environments (for example, the gastrointestinal tract lumen), Shigella is primed for invasion and expresses extended T3SS needles while reducing Ipa (invasion plasmid antigen) effector secretion. This is mediated by FNR (fumarate and nitrate reduction), a regulator of anaerobic metabolism that represses transcription of spa32 and spa33, virulence genes that regulate secretion through the T3SS. We demonstrate there is a zone of relative oxygenation adjacent to the gastrointestinal tract mucosa, caused by diffusion from the capillary network at the tips of villi. This would reverse the anaerobic block of Ipa secretion, allowing T3SS activation at its precise site of action, enhancing invasion and virulence.

U2 - 10.1038/nature08970

DO - 10.1038/nature08970

M3 - Article

VL - 465

SP - 355-U113

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7296

ER -