Modular off-chip emulsion generator enabled by a revolving needle

Research output: Contribution to journalArticlepeer-review

Authors

  • Yuxin Zhang
  • Qianbin Zhao
  • Dan Yuan
  • Hangrui Liu
  • Guolin Yun
  • Hongda Lu
  • Ming Li
  • Jinhong Guo
  • Weihua Li

External organisations

  • University of Wollongong (UOW)
  • Macquarie University
  • University of Electronic Science and Technology of China

Abstract

Microfluidic chips have demonstrated unparalleled abilities in droplet generation, including precise control over droplet size and monodispersity. And yet, their rather complicated microfabrication process and operation can be a barrier for inexperienced researchers, which hinders microdroplets from unleashing their potential in broader fields of research. Here, we attempt to remove this barrier by developing an integrated and modular revolving needle emulsion generator (RNEG) to achieve high-throughput production of uniformly sized droplets in an off-chip manner. The RNEG works by driving a revolving needle to pinch the dispersed phase in a minicentrifuge tube. The system is constructed using modular components without involving any microfabrication, thereby enabling user-friendly operation. The RNEG is capable of producing microdroplets of various liquids with diameters ranging from tens to hundreds of micrometres. We further examine the principle of operation using numerical simulations and establish a simple model to predict the droplet size. Moreover, by integrating curing and centrifugation processes, the RNEG can produce hydrogel microparticles and transfer them from an oil phase into a water phase. Using this ability, we demonstrate the encapsulation and culture of single yeast cells within hydrogel microparticles. We envisage that the RNEG can become a versatile and powerful tool for high-throughput production of emulsions to facilitate diverse biological and chemical research.

Details

Original languageEnglish
Pages (from-to)4592-4599
Number of pages8
JournalLab on a Chip
Volume20
Issue number24
Early online date28 Oct 2020
Publication statusPublished - 15 Dec 2020