Mighty small: Observing and modeling individual microbes becomes big science

Research output: Contribution to journalComment/debatepeer-review

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Mighty small : Observing and modeling individual microbes becomes big science. / Kreft, Jan Ulrich; Plugge, Caroline M.; Grimm, Volker; Prats, Clara; Leveau, Johan H J; Banitz, Thomas; Baines, Stephen; Clark, James; Ros, Alexandra; Klapper, Isaac; Topping, Chris J.; Field, Anthony J.; Schuler, Andrew; Litchman, Elena; Hellweger, Ferdi L.

In: National Academy of Sciences. Proceedings, Vol. 110, No. 45, 05.11.2013, p. 18027-18028.

Research output: Contribution to journalComment/debatepeer-review

Harvard

Kreft, JU, Plugge, CM, Grimm, V, Prats, C, Leveau, JHJ, Banitz, T, Baines, S, Clark, J, Ros, A, Klapper, I, Topping, CJ, Field, AJ, Schuler, A, Litchman, E & Hellweger, FL 2013, 'Mighty small: Observing and modeling individual microbes becomes big science', National Academy of Sciences. Proceedings, vol. 110, no. 45, pp. 18027-18028. https://doi.org/10.1073/pnas.1317472110

APA

Kreft, J. U., Plugge, C. M., Grimm, V., Prats, C., Leveau, J. H. J., Banitz, T., Baines, S., Clark, J., Ros, A., Klapper, I., Topping, C. J., Field, A. J., Schuler, A., Litchman, E., & Hellweger, F. L. (2013). Mighty small: Observing and modeling individual microbes becomes big science. National Academy of Sciences. Proceedings, 110(45), 18027-18028. https://doi.org/10.1073/pnas.1317472110

Vancouver

Author

Kreft, Jan Ulrich ; Plugge, Caroline M. ; Grimm, Volker ; Prats, Clara ; Leveau, Johan H J ; Banitz, Thomas ; Baines, Stephen ; Clark, James ; Ros, Alexandra ; Klapper, Isaac ; Topping, Chris J. ; Field, Anthony J. ; Schuler, Andrew ; Litchman, Elena ; Hellweger, Ferdi L. / Mighty small : Observing and modeling individual microbes becomes big science. In: National Academy of Sciences. Proceedings. 2013 ; Vol. 110, No. 45. pp. 18027-18028.

Bibtex

@article{bb712fa9533f493d8435e36385132fd7,
title = "Mighty small: Observing and modeling individual microbes becomes big science",
abstract = "Progress in microbiology has always been driven by technological advances, ever since Antonie van Leeuwenhoek discovered bacteria by making an improved compound microscope. However, until very recently we have not been able to identify microbes and record their mostly invisible activities, such as nutrient consumption or toxin production on the level of the single cell, not even in the laboratory. This is now changing with the rapid rise of exciting new technologies for single-cell microbiology (1, 2), which enable microbiologists to do what plant and animal ecologists have been doing for a long time: observe who does what, when, where, and next to whom. Single cells taken from the environment can be identified and even their genomes sequenced. Ex situ, their size, elemental, and biochemical composition, as well as other characteristics can be measured with high-throughput and cells sorted accordingly. Even better, individual microbes can be observed in situ with a range of novel microscopic and spectroscopic methods, enabling localization, identification, or functional characterization of cells in a natural sample, combined with detecting uptake of labeled compounds. Alternatively, they can be placed into fabricated microfluidic environments, where they can be positioned, exposed to stimuli, monitored, and their interactions controlled “in microfluido.” By introducing genetically engineered reporter cells into a fabricated landscape or a microcosm taken from nature, their reproductive success or activity can be followed, or their sensing of their local environment recorded.",
author = "Kreft, {Jan Ulrich} and Plugge, {Caroline M.} and Volker Grimm and Clara Prats and Leveau, {Johan H J} and Thomas Banitz and Stephen Baines and James Clark and Alexandra Ros and Isaac Klapper and Topping, {Chris J.} and Field, {Anthony J.} and Andrew Schuler and Elena Litchman and Hellweger, {Ferdi L.}",
year = "2013",
month = nov,
day = "5",
doi = "10.1073/pnas.1317472110",
language = "English",
volume = "110",
pages = "18027--18028",
journal = "Proceedings of the National Academy of Sciences",
issn = "1091-6490",
publisher = "National Academy of Sciences",
number = "45",

}

RIS

TY - JOUR

T1 - Mighty small

T2 - Observing and modeling individual microbes becomes big science

AU - Kreft, Jan Ulrich

AU - Plugge, Caroline M.

AU - Grimm, Volker

AU - Prats, Clara

AU - Leveau, Johan H J

AU - Banitz, Thomas

AU - Baines, Stephen

AU - Clark, James

AU - Ros, Alexandra

AU - Klapper, Isaac

AU - Topping, Chris J.

AU - Field, Anthony J.

AU - Schuler, Andrew

AU - Litchman, Elena

AU - Hellweger, Ferdi L.

PY - 2013/11/5

Y1 - 2013/11/5

N2 - Progress in microbiology has always been driven by technological advances, ever since Antonie van Leeuwenhoek discovered bacteria by making an improved compound microscope. However, until very recently we have not been able to identify microbes and record their mostly invisible activities, such as nutrient consumption or toxin production on the level of the single cell, not even in the laboratory. This is now changing with the rapid rise of exciting new technologies for single-cell microbiology (1, 2), which enable microbiologists to do what plant and animal ecologists have been doing for a long time: observe who does what, when, where, and next to whom. Single cells taken from the environment can be identified and even their genomes sequenced. Ex situ, their size, elemental, and biochemical composition, as well as other characteristics can be measured with high-throughput and cells sorted accordingly. Even better, individual microbes can be observed in situ with a range of novel microscopic and spectroscopic methods, enabling localization, identification, or functional characterization of cells in a natural sample, combined with detecting uptake of labeled compounds. Alternatively, they can be placed into fabricated microfluidic environments, where they can be positioned, exposed to stimuli, monitored, and their interactions controlled “in microfluido.” By introducing genetically engineered reporter cells into a fabricated landscape or a microcosm taken from nature, their reproductive success or activity can be followed, or their sensing of their local environment recorded.

AB - Progress in microbiology has always been driven by technological advances, ever since Antonie van Leeuwenhoek discovered bacteria by making an improved compound microscope. However, until very recently we have not been able to identify microbes and record their mostly invisible activities, such as nutrient consumption or toxin production on the level of the single cell, not even in the laboratory. This is now changing with the rapid rise of exciting new technologies for single-cell microbiology (1, 2), which enable microbiologists to do what plant and animal ecologists have been doing for a long time: observe who does what, when, where, and next to whom. Single cells taken from the environment can be identified and even their genomes sequenced. Ex situ, their size, elemental, and biochemical composition, as well as other characteristics can be measured with high-throughput and cells sorted accordingly. Even better, individual microbes can be observed in situ with a range of novel microscopic and spectroscopic methods, enabling localization, identification, or functional characterization of cells in a natural sample, combined with detecting uptake of labeled compounds. Alternatively, they can be placed into fabricated microfluidic environments, where they can be positioned, exposed to stimuli, monitored, and their interactions controlled “in microfluido.” By introducing genetically engineered reporter cells into a fabricated landscape or a microcosm taken from nature, their reproductive success or activity can be followed, or their sensing of their local environment recorded.

UR - http://www.scopus.com/inward/record.url?scp=84887289464&partnerID=8YFLogxK

U2 - 10.1073/pnas.1317472110

DO - 10.1073/pnas.1317472110

M3 - Comment/debate

C2 - 24194530

AN - SCOPUS:84887289464

VL - 110

SP - 18027

EP - 18028

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 45

ER -