Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines

Research output: Contribution to journalArticlepeer-review

Standard

Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines. / Hansjosten, Iris; Rapp, Juliane; Reiner, Luisa; Vatter, Ruben; Fritsch-Decker, Susanne; Peravali, Ravindra; Palosaari, Taina; Joossens, Elisabeth; Gerloff, Kirsten; Macko, Peter; Whelan, Maurice; Gilliland, Douglas; Ojea-Jimenez, Isaac; Monopoli, Marco P; Rocks, Louise; Garry, David; Dawson, Kenneth; Röttgermann, Peter J F; Murschhauser, Alexandra; Rädler, Joachim O; Tang, Selina V Y; Gooden, Pete; Belinga-Desaunay, Marie-France A; Khan, Abdullah O; Briffa, Sophie; Guggenheim, Emily; Papadiamantis, Anastasios; Lynch, Iseult; Valsami-Jones, Eugenia; Diabaté, Silvia; Weiss, Carsten.

In: Archives of toxicology, Vol. 92, No. 2, 01.02.2018, p. 633-649.

Research output: Contribution to journalArticlepeer-review

Harvard

Hansjosten, I, Rapp, J, Reiner, L, Vatter, R, Fritsch-Decker, S, Peravali, R, Palosaari, T, Joossens, E, Gerloff, K, Macko, P, Whelan, M, Gilliland, D, Ojea-Jimenez, I, Monopoli, MP, Rocks, L, Garry, D, Dawson, K, Röttgermann, PJF, Murschhauser, A, Rädler, JO, Tang, SVY, Gooden, P, Belinga-Desaunay, M-FA, Khan, AO, Briffa, S, Guggenheim, E, Papadiamantis, A, Lynch, I, Valsami-Jones, E, Diabaté, S & Weiss, C 2018, 'Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines', Archives of toxicology, vol. 92, no. 2, pp. 633-649. https://doi.org/10.1007/s00204-017-2106-7

APA

Hansjosten, I., Rapp, J., Reiner, L., Vatter, R., Fritsch-Decker, S., Peravali, R., Palosaari, T., Joossens, E., Gerloff, K., Macko, P., Whelan, M., Gilliland, D., Ojea-Jimenez, I., Monopoli, M. P., Rocks, L., Garry, D., Dawson, K., Röttgermann, P. J. F., Murschhauser, A., ... Weiss, C. (2018). Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines. Archives of toxicology, 92(2), 633-649. https://doi.org/10.1007/s00204-017-2106-7

Vancouver

Author

Hansjosten, Iris ; Rapp, Juliane ; Reiner, Luisa ; Vatter, Ruben ; Fritsch-Decker, Susanne ; Peravali, Ravindra ; Palosaari, Taina ; Joossens, Elisabeth ; Gerloff, Kirsten ; Macko, Peter ; Whelan, Maurice ; Gilliland, Douglas ; Ojea-Jimenez, Isaac ; Monopoli, Marco P ; Rocks, Louise ; Garry, David ; Dawson, Kenneth ; Röttgermann, Peter J F ; Murschhauser, Alexandra ; Rädler, Joachim O ; Tang, Selina V Y ; Gooden, Pete ; Belinga-Desaunay, Marie-France A ; Khan, Abdullah O ; Briffa, Sophie ; Guggenheim, Emily ; Papadiamantis, Anastasios ; Lynch, Iseult ; Valsami-Jones, Eugenia ; Diabaté, Silvia ; Weiss, Carsten. / Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines. In: Archives of toxicology. 2018 ; Vol. 92, No. 2. pp. 633-649.

Bibtex

@article{db131b92ccf647d48b2f1ab0d001eadd,
title = "Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines",
abstract = "Manufactured nanomaterials (MNMs) selected from a library of over 120 different MNMs with varied compositions, sizes, and surface coatings were tested by four different laboratories for toxicity by high-throughput/-content (HT/C) techniques. The selected particles comprise 14 MNMs composed of CeO2, Ag, TiO2, ZnO and SiO2with different coatings and surface characteristics at varying concentrations. The MNMs were tested in different mammalian cell lines at concentrations between 0.5 and 250 µg/mL to link physical-chemical properties to multiple adverse effects. The cell lines are derived from relevant organs such as liver, lung, colon and the immune system. Endpoints such as viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential, lysosomal acidification and steatosis have been studied. Soluble MNMs, Ag and ZnO, were toxic in all cell types. TiO2and SiO2MNMs also triggered toxicity in some, but not all, cell types and the cell type-specific effects were influenced by the specific coating and surface modification. CeO2MNMs were nearly ineffective in our test systems. Differentiated liver cells appear to be most sensitive to MNMs, Whereas most of the investigated MNMs showed no acute toxicity, it became clear that some show adverse effects dependent on the assay and cell line. Hence, it is advised that future nanosafety studies utilise a multi-parametric approach such as HT/C screening to avoid missing signs of toxicity. Furthermore, some of the cell type-specific effects should be followed up in more detail and might also provide an incentive to address potential adverse effects in vivo in the relevant organ.",
keywords = "Journal Article, maufactured nanomaterials , toxicity , high-throughput screening , cell type specificity , cell death , adverse outcome pathways , nanosafety",
author = "Iris Hansjosten and Juliane Rapp and Luisa Reiner and Ruben Vatter and Susanne Fritsch-Decker and Ravindra Peravali and Taina Palosaari and Elisabeth Joossens and Kirsten Gerloff and Peter Macko and Maurice Whelan and Douglas Gilliland and Isaac Ojea-Jimenez and Monopoli, {Marco P} and Louise Rocks and David Garry and Kenneth Dawson and R{\"o}ttgermann, {Peter J F} and Alexandra Murschhauser and R{\"a}dler, {Joachim O} and Tang, {Selina V Y} and Pete Gooden and Belinga-Desaunay, {Marie-France A} and Khan, {Abdullah O} and Sophie Briffa and Emily Guggenheim and Anastasios Papadiamantis and Iseult Lynch and Eugenia Valsami-Jones and Silvia Diabat{\'e} and Carsten Weiss",
year = "2018",
month = feb,
day = "1",
doi = "10.1007/s00204-017-2106-7",
language = "English",
volume = "92",
pages = "633--649",
journal = "Archives of toxicology",
issn = "0340-5761",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines

AU - Hansjosten, Iris

AU - Rapp, Juliane

AU - Reiner, Luisa

AU - Vatter, Ruben

AU - Fritsch-Decker, Susanne

AU - Peravali, Ravindra

AU - Palosaari, Taina

AU - Joossens, Elisabeth

AU - Gerloff, Kirsten

AU - Macko, Peter

AU - Whelan, Maurice

AU - Gilliland, Douglas

AU - Ojea-Jimenez, Isaac

AU - Monopoli, Marco P

AU - Rocks, Louise

AU - Garry, David

AU - Dawson, Kenneth

AU - Röttgermann, Peter J F

AU - Murschhauser, Alexandra

AU - Rädler, Joachim O

AU - Tang, Selina V Y

AU - Gooden, Pete

AU - Belinga-Desaunay, Marie-France A

AU - Khan, Abdullah O

AU - Briffa, Sophie

AU - Guggenheim, Emily

AU - Papadiamantis, Anastasios

AU - Lynch, Iseult

AU - Valsami-Jones, Eugenia

AU - Diabaté, Silvia

AU - Weiss, Carsten

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Manufactured nanomaterials (MNMs) selected from a library of over 120 different MNMs with varied compositions, sizes, and surface coatings were tested by four different laboratories for toxicity by high-throughput/-content (HT/C) techniques. The selected particles comprise 14 MNMs composed of CeO2, Ag, TiO2, ZnO and SiO2with different coatings and surface characteristics at varying concentrations. The MNMs were tested in different mammalian cell lines at concentrations between 0.5 and 250 µg/mL to link physical-chemical properties to multiple adverse effects. The cell lines are derived from relevant organs such as liver, lung, colon and the immune system. Endpoints such as viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential, lysosomal acidification and steatosis have been studied. Soluble MNMs, Ag and ZnO, were toxic in all cell types. TiO2and SiO2MNMs also triggered toxicity in some, but not all, cell types and the cell type-specific effects were influenced by the specific coating and surface modification. CeO2MNMs were nearly ineffective in our test systems. Differentiated liver cells appear to be most sensitive to MNMs, Whereas most of the investigated MNMs showed no acute toxicity, it became clear that some show adverse effects dependent on the assay and cell line. Hence, it is advised that future nanosafety studies utilise a multi-parametric approach such as HT/C screening to avoid missing signs of toxicity. Furthermore, some of the cell type-specific effects should be followed up in more detail and might also provide an incentive to address potential adverse effects in vivo in the relevant organ.

AB - Manufactured nanomaterials (MNMs) selected from a library of over 120 different MNMs with varied compositions, sizes, and surface coatings were tested by four different laboratories for toxicity by high-throughput/-content (HT/C) techniques. The selected particles comprise 14 MNMs composed of CeO2, Ag, TiO2, ZnO and SiO2with different coatings and surface characteristics at varying concentrations. The MNMs were tested in different mammalian cell lines at concentrations between 0.5 and 250 µg/mL to link physical-chemical properties to multiple adverse effects. The cell lines are derived from relevant organs such as liver, lung, colon and the immune system. Endpoints such as viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential, lysosomal acidification and steatosis have been studied. Soluble MNMs, Ag and ZnO, were toxic in all cell types. TiO2and SiO2MNMs also triggered toxicity in some, but not all, cell types and the cell type-specific effects were influenced by the specific coating and surface modification. CeO2MNMs were nearly ineffective in our test systems. Differentiated liver cells appear to be most sensitive to MNMs, Whereas most of the investigated MNMs showed no acute toxicity, it became clear that some show adverse effects dependent on the assay and cell line. Hence, it is advised that future nanosafety studies utilise a multi-parametric approach such as HT/C screening to avoid missing signs of toxicity. Furthermore, some of the cell type-specific effects should be followed up in more detail and might also provide an incentive to address potential adverse effects in vivo in the relevant organ.

KW - Journal Article

KW - maufactured nanomaterials

KW - toxicity

KW - high-throughput screening

KW - cell type specificity

KW - cell death

KW - adverse outcome pathways

KW - nanosafety

U2 - 10.1007/s00204-017-2106-7

DO - 10.1007/s00204-017-2106-7

M3 - Article

C2 - 29119250

VL - 92

SP - 633

EP - 649

JO - Archives of toxicology

JF - Archives of toxicology

SN - 0340-5761

IS - 2

ER -