microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells

E Vigorito; KL Perks; C Abreu-Goodger; S Bunting; Z Xiang; S Kohlhaas; PP Das; EA Miska; A Rodriguez; A Bradley; KG Smith; C Rada; AJ Enright; Kai-Michael Toellner; Ian MacLennan; M Turner

doi:10.1016/j.immuni.2007.10.009

microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells

E Vigorito, KL Perks, C Abreu-Goodger, S Bunting, Z Xiang, S Kohlhaas, PP Das, EA Miska, A Rodriguez, A Bradley, KG Smith, C Rada, AJ Enright, Kai-Michael Toellner, Ian MacLennan, M Turner

Immunology and Immunotherapy

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Abstract

microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.

Original language	English
Pages (from-to)	847-59
Number of pages	13
Journal	Immunity
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.immuni.2007.10.009
Publication status	Published - 21 Dec 2007

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Vigorito, E., Perks, KL., Abreu-Goodger, C., Bunting, S., Xiang, Z., Kohlhaas, S., Das, PP., Miska, EA., Rodriguez, A., Bradley, A., Smith, KG., Rada, C., Enright, AJ., Toellner, K-M., MacLennan, I., & Turner, M. (2007). microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells. Immunity, 27(6), 847-59. https://doi.org/10.1016/j.immuni.2007.10.009

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title = "microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells",

abstract = "microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.",

author = "E Vigorito and KL Perks and C Abreu-Goodger and S Bunting and Z Xiang and S Kohlhaas and PP Das and EA Miska and A Rodriguez and A Bradley and KG Smith and C Rada and AJ Enright and Kai-Michael Toellner and Ian MacLennan and M Turner",

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Vigorito, E, Perks, KL, Abreu-Goodger, C, Bunting, S, Xiang, Z, Kohlhaas, S, Das, PP, Miska, EA, Rodriguez, A, Bradley, A, Smith, KG, Rada, C, Enright, AJ, Toellner, K-M, MacLennan, I & Turner, M 2007, 'microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells', Immunity, vol. 27, no. 6, pp. 847-59. https://doi.org/10.1016/j.immuni.2007.10.009

TY - JOUR

T1 - microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells

AU - Vigorito, E

AU - Perks, KL

AU - Abreu-Goodger, C

AU - Bunting, S

AU - Xiang, Z

AU - Kohlhaas, S

AU - Das, PP

AU - Miska, EA

AU - Rodriguez, A

AU - Bradley, A

AU - Smith, KG

AU - Rada, C

AU - Enright, AJ

AU - Toellner, Kai-Michael

AU - MacLennan, Ian

AU - Turner, M

PY - 2007/12/21

Y1 - 2007/12/21

N2 - microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.

AB - microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.

U2 - 10.1016/j.immuni.2007.10.009

DO - 10.1016/j.immuni.2007.10.009

M3 - Article

C2 - 18055230

SN - 1097-4180

VL - 27

SP - 847

EP - 859

JO - Immunity

JF - Immunity

IS - 6

ER -

microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells

Abstract

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