MicroRNA Signature of Traumatic Brain Injury: From the Biomarker Discovery to the Point-of-Care

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MicroRNA Signature of Traumatic Brain Injury: From the Biomarker Discovery to the Point-of-Care. / Di Pietro, Valentina; Yakoub, Kamal; Scarpa, Ugo; Di Pietro, Cinza; Belli, Antonio.

In: Frontiers in neurology, Vol. 9, No. 429, 429, 14.06.2018.

Research output: Contribution to journalReview article

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@article{8800ced1d08142a380dc38eedf290b3d,
title = "MicroRNA Signature of Traumatic Brain Injury:: From the Biomarker Discovery to the Point-of-Care",
abstract = "Traumatic brain injury (TBI) is a serious problem that causes high morbidity and mortality around the world. Currently, no reliable biomarkers are used to assess the severity and predict the recovery. Many protein biomarkers were extensively studied for diagnosis and prognosis of different TBI severities such as S-100β, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), neurofilament light chain (NFL), cleaved tau protein (C-tau), and ubiquitin C-terminal hydrolase-L1 (UCH-L1). However, none of these candidates is currently used in the clinical practice, due to relatively low sensitivity, for the diagnosis of mild TBI (mTBI) or mild to moderate TBI (MMTBI) patients who are clinically well and do not have a detectable intracranial pathology on the scans. MicroRNAs (miRNAs or miRs) are a class of small endogenous molecular regulators, which showed to be altered in different pathologies, including TBI and for this reason, their potential role in diagnosis, prognosis and therapeutic applications, is explored. Promising miRNAs such as miR-21, miR-16 or let-7i were identified as suitable candidate biomarkers for TBI and can differentiate mild from severe TBI. Also, they might represent new potential therapeutic targets. Identification of miRNA signature in tissue or biofluids, for several pathological conditions, is now possible thanks to the introduction of new high-throughput technologies such as microarray platform, Nanostring technologies or Next Generation Sequencing. This review has the aim to describe the role of microRNA in TBI and to explore the most commonly used techniques to identify microRNA profile. Understanding the strengths and limitations of the different methods can aid in the practical use of miRNA profiling for diverse clinical applications, including the development of a point-of-care device.",
keywords = "traumatic brain injury, biomarkers, microRNA, diagnosis, prognosis, therapy, high-throughput technology, point-of-care",
author = "{Di Pietro}, Valentina and Kamal Yakoub and Ugo Scarpa and {Di Pietro}, Cinza and Antonio Belli",
year = "2018",
month = "6",
day = "14",
doi = "10.3389/fneur.2018.00429",
language = "English",
volume = "9",
journal = "Frontiers in neurology",
issn = "1664-2295",
publisher = "Frontiers",
number = "429",

}

RIS

TY - JOUR

T1 - MicroRNA Signature of Traumatic Brain Injury:

T2 - From the Biomarker Discovery to the Point-of-Care

AU - Di Pietro, Valentina

AU - Yakoub, Kamal

AU - Scarpa, Ugo

AU - Di Pietro, Cinza

AU - Belli, Antonio

PY - 2018/6/14

Y1 - 2018/6/14

N2 - Traumatic brain injury (TBI) is a serious problem that causes high morbidity and mortality around the world. Currently, no reliable biomarkers are used to assess the severity and predict the recovery. Many protein biomarkers were extensively studied for diagnosis and prognosis of different TBI severities such as S-100β, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), neurofilament light chain (NFL), cleaved tau protein (C-tau), and ubiquitin C-terminal hydrolase-L1 (UCH-L1). However, none of these candidates is currently used in the clinical practice, due to relatively low sensitivity, for the diagnosis of mild TBI (mTBI) or mild to moderate TBI (MMTBI) patients who are clinically well and do not have a detectable intracranial pathology on the scans. MicroRNAs (miRNAs or miRs) are a class of small endogenous molecular regulators, which showed to be altered in different pathologies, including TBI and for this reason, their potential role in diagnosis, prognosis and therapeutic applications, is explored. Promising miRNAs such as miR-21, miR-16 or let-7i were identified as suitable candidate biomarkers for TBI and can differentiate mild from severe TBI. Also, they might represent new potential therapeutic targets. Identification of miRNA signature in tissue or biofluids, for several pathological conditions, is now possible thanks to the introduction of new high-throughput technologies such as microarray platform, Nanostring technologies or Next Generation Sequencing. This review has the aim to describe the role of microRNA in TBI and to explore the most commonly used techniques to identify microRNA profile. Understanding the strengths and limitations of the different methods can aid in the practical use of miRNA profiling for diverse clinical applications, including the development of a point-of-care device.

AB - Traumatic brain injury (TBI) is a serious problem that causes high morbidity and mortality around the world. Currently, no reliable biomarkers are used to assess the severity and predict the recovery. Many protein biomarkers were extensively studied for diagnosis and prognosis of different TBI severities such as S-100β, glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), neurofilament light chain (NFL), cleaved tau protein (C-tau), and ubiquitin C-terminal hydrolase-L1 (UCH-L1). However, none of these candidates is currently used in the clinical practice, due to relatively low sensitivity, for the diagnosis of mild TBI (mTBI) or mild to moderate TBI (MMTBI) patients who are clinically well and do not have a detectable intracranial pathology on the scans. MicroRNAs (miRNAs or miRs) are a class of small endogenous molecular regulators, which showed to be altered in different pathologies, including TBI and for this reason, their potential role in diagnosis, prognosis and therapeutic applications, is explored. Promising miRNAs such as miR-21, miR-16 or let-7i were identified as suitable candidate biomarkers for TBI and can differentiate mild from severe TBI. Also, they might represent new potential therapeutic targets. Identification of miRNA signature in tissue or biofluids, for several pathological conditions, is now possible thanks to the introduction of new high-throughput technologies such as microarray platform, Nanostring technologies or Next Generation Sequencing. This review has the aim to describe the role of microRNA in TBI and to explore the most commonly used techniques to identify microRNA profile. Understanding the strengths and limitations of the different methods can aid in the practical use of miRNA profiling for diverse clinical applications, including the development of a point-of-care device.

KW - traumatic brain injury

KW - biomarkers

KW - microRNA

KW - diagnosis

KW - prognosis

KW - therapy

KW - high-throughput technology

KW - point-of-care

U2 - 10.3389/fneur.2018.00429

DO - 10.3389/fneur.2018.00429

M3 - Review article

C2 - 29963002

VL - 9

JO - Frontiers in neurology

JF - Frontiers in neurology

SN - 1664-2295

IS - 429

M1 - 429

ER -